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Plasmid#184867PurposepSwap plasmid part with insert fragment moduleDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTOX3
Plasmid#127448PurposeEncodes Tse2 toxin. CAM-RDepositorInserttse2 toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFJ108 - pENTR221 - [cbr-unc-119(+)]
Plasmid#200367Purposecbr-unc-119 rescue gene for use as co-injection marker.DepositorInsert[cbr-unc-119(+)]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV0679
Plasmid#149689PurposeBacterial expression of SENP_EuH proteaseDepositorInsertSENP_EuH protease
UseTagsHis14-tag and Tev protease cleavage siteExpressionBacterialMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEft-3::oxCerulean-oxVenus::Lgg-1
Plasmid#68040PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed ubiquitously, for monitoring autophagyDepositorInsertpEft-3::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR Lck-TagBFP
Plasmid#171034PurposeTagBFP-tagged Lck kinaseDepositorInsertLck
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-Ferritin-Dronpa
Plasmid#101194PurposeExpresses a Ferritin-Dronpa fusion construct in E. coliDepositorInsertFerritin-Dronpa
UseTags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJA291
Plasmid#74487PurposeeF1alpha::puroR::mCherry::T2A::eGFP::MCS::wPRE for assaying effects of C-terminal fusions to eGFPDepositorInsertpuroR-mCherry-T2A-eGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSWAP_Entry_HA
Plasmid#184866PurposepSwap plasmid part with HA homology armsDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSWAP_Entry_HB
Plasmid#184868PurposepSwap plasmid part with HB homology armsDepositorInsertlacZalpha,ccdB
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRL277
Plasmid#70255PurposesacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.DepositorTypeEmpty backboneUseConditionally lethalTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRO16Sh2
Plasmid#180604PurposeExpression of ShCAST under control of Plac with pRO1600 origin.DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLacAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC1823_pCR8-RBFOX1N-dCasRx-C
Plasmid#118657PurposeGateway Donor; RBFOX1N-dCasRx-C ORF; No promoterDepositorInsertRBFOX1N-dCasRx-C
UseCRISPRTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF1-VP16
Plasmid#51426PurposeArtificial transcription factor with VP16DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSNAP-macroH2A1.2 Zeo
Plasmid#132428PurposeExpressing SNAP-tagged histone macroH2A1.2 in mammalian cellsDepositorInsertmacroH2A1.2
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p155 pCMV-EGFP/beta-gal
Plasmid#8387DepositorInsertfloxed EGFP
UseCre/Lox; Beta-galTagsExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
lenti-fullef1a-FAKSPARK2
Plasmid#195265Purposeexpress wtFAKSPARK2 in a lenti vectorDepositorInsertFAKSPARK2 sensor
UseLentiviralTagsExpressionMammalianMutationPromoteref1aAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTOX1
Plasmid#127446PurposeEncodes YhaV toxin. CAM-RDepositorInsertyhaV toxin
UseTagsExpressionBacterialMutationPromoterpRhaAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2G1
Plasmid#15790DepositorInsertUbiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion eGFP
Plasmid#112848PurposeGeneral donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertEGFP
UseUnspecified; General donorTagsExpressionMutationPromoterNoAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJH021_SC101_pLAC_Cas12c2_Kan
Plasmid#183072PurposeWild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HO-pGAL-poly-KanMX4-HO
Plasmid#51664Purposeyeast plasmid for integration of target sequence at HO locus, Kan selection, pGAL inducible promoterDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSP64-Vasa-GFP
Plasmid#135595PurposeExpression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratusDepositorInsertVasa-GFP
UseIn vitro transcription (mrna synthesis)TagsEGFPExpressionMutationPromoterSP6Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEA106
Plasmid#187872PurposeExpresses mCherry under the virB promoter.DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAgrobacterium virB promoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
p133 pSV-STOP-luc
Plasmid#8390DepositorInsertfloxed STOP
UseCre/Lox and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAC1825_pCR8-dCasRx-RBM38
Plasmid#118662PurposeGateway Donor; dCasRx-RBM38 ORF; No promoterDepositorInsertdCasRx-RBM38
UseCRISPRTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDAI-SceI
Plasmid#32061PurposeConstruction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonucleaseDepositorInsertI-SceI nuclease
UseTagsExpressionBacterialMutationPromoterPdhfrAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC201plus
Plasmid#164227PurposeIntegrative plasmid to introduce modified rhamnose-inducible promoter into bacterial genomes. Harbours enhanced rhaS binding site and encodes T7 gene10 stem loop in mRNA for enhanced expressionDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterRhamnose-inducibleAvailable sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
eNOS S1179D
Plasmid#22484DepositorInserteNOS S1179D
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
Plasmid#140153PurposeExpresses a regulatable vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJA327
Plasmid#74486Purposesuperfolder GFP (myo-3::sfGFP::let-858, using backbone as in L3785)DepositorInsertsuperfolder GFP
UseTagsExpressionWormMutationPromotermyo-3Available sinceApril 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC1804_pmax-dCasRx-RBM38
Plasmid#118638PurposeTransient transfection; Expresses dCasRx-RBM38; CAGGS promoterDepositorInsertdCasRx-RBM38
UseCRISPRTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCJH019_SC101_pLAC_Cas12c(4)_Kan
Plasmid#183071PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertCas12c_4
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcNB2
Plasmid#89242PurposeResurfaced Cell-penetrating 5F7 nanobodyDepositorInsertpcNB2 (5F7)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2T
Plasmid#15808DepositorInsertUbiquitin conjugating enzyme UbE2T (UBE2T Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-wtVHL
Plasmid#13390DepositorInsertvon Hippel-Lindau (Vhl Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hSV1_WT
Plasmid#129483PurposeMammalian expression of full-length human supervillin, isoform 1.DepositorInsertSupervillin (SVIL Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCS2+ Cas9
Plasmid#122948PurposeFor in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryosDepositorInsertCas9
UseSynthetic BiologyTagsExpressionMutationPromoterSP6Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T
Plasmid#64954Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS405 VAC8-GFP
Plasmid#166836PurposeExpresses Vac8-GFP in yeasts.DepositorInsertVAC8 (VAC8 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterVAC8 promoterAvailable sinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGCDNsam-Foxa1
Plasmid#33003DepositorInsertforkhead box A1 (Foxa1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsTNRC6Biso1
Plasmid#25034DepositorInsertTNRC6B (TNRC6B Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
CKAR-T/A
Plasmid#14861DepositorInsertC kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationT/APromoterAvailable sinceAug. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2E3
Plasmid#15789DepositorInsertUbiquitin conjugating enzyme UbE2E3 (UBE2E3 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion mAID-mCherry with Neo
Plasmid#113831PurposeEasyFusion vector for targeting endogeneous genes with mAID-mCherryDepositorInsertmAID-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCSh5vio-2300
Plasmid#170987PurposeEncodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.DepositorInsertSh-CAST sgRNA 2300 violacein
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterplacIAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP704nano
Plasmid#206192PurposeHuman alpha-tubulin labelled with near-infrared fluorescent protein miRFP704nanoDepositorInsertmiRFP704nano-human alpha-tubulin
UseTagsmiRFP704nanoExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-DHFR-myc
Plasmid#118852PurposeFor fluorophore translocation assay and inducible tagging with zapalog.DepositorTypeEmpty backboneUseTagsDHFR, Myc, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only