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Showing: 6761 - 6780 of 7946 results

  3. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

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  6. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

    Plasmid
    #99692
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (Hbb Synthetic)
    Use
    AAV
    Tags
    VPR mini
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    99692-plasmid-map-sequence-id-194594

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  9. pdCASclos

    Plasmid
    #73640
    Purpose
    Transcriptional repression for gene Spo0A (CAC-2011) in Clostridium acetobutylicum ATCC 824
    Depositor
    Sheng Yang
    Article
    Li et al Biotechnol J. 2016 May 23. doi: 10.1002/
    Inserts
    dCas9
    sgRNA to spo0A
    Use
    E.coli-clostridium shuttle vector
    Tags
    Expression
    Mutation
    D10A, H840A
    Promoter
    Pj23119 and ptb
    Available since
    June 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73640-plasmid-map-sequence-id-148604

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  10. pUF1-dCas9-GCN5

    Plasmid
    #122509
    Purpose
    Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Article
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Insert
    dCas9-GCN5
    Use
    CRISPR
    Tags
    3*FLAG
    Expression
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122509-plasmid-map-sequence-id-245888

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  11. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

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  13. qTAG-C-mNeon-Puro-TOMM20

    Plasmid
    #207790
    Purpose
    Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Insert
    TOMM20 Homology Arms flanking a mNeon-Puro Cassette (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    207790-plasmid-map-sequence-id-415639

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  18. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid
    #104438
    Purpose
    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;
    Depositor
    Detlef Weigel
    Article
    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Plant
    Mutation
    Promoter
    UBQ10
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104438-plasmid-map-sequence-id-201144

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  19. pUF1-dCas9-Sir2a

    Plasmid
    #122510
    Purpose
    Combining with the specific sgRNA sequence to repress the Plasmodium falciparum endogenous gene transcription, through the decreasing of H3K9 acetylation.
    Depositor
    Lubin Jiang
    Article
    Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116
    Insert
    dCas9-Sir2a
    Use
    CRISPR
    Tags
    3*FLAG
    Expression
    Mutation
    dCas9(D10A,H840A)
    Promoter
    PfHsp86
    Available since
    June 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    122510-plasmid-map-sequence-id-243592

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Showing: 6761 - 6780 of 7946 results