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Plasmid#102994PurposeExpresses histone fused GCaMP6f under the synapsin promoterDepositorInsertH2B-GCaMP6f
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin I promoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-Flag-Fbxl3
Plasmid#48751PurposeExpression of FLAG-tagged Fbxl3DepositorInsertFbxl3 (Fbxl3 Mouse)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-9-5p
Plasmid#103749PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-9-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-9-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSico_U6-PLOD2 sgRNA4
Plasmid#136459PurposeExpression of gRNA against human PLOD2DepositorInsertgRNA against human PLOD2 (PLOD2 Human, Synthetic)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Aps-SH2
Plasmid#46404DepositorInsertAps (SH2B2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 404-523PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(N)-SH2
Plasmid#46520DepositorInsertSyk(N) (SYK Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-114PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX ShcA-SH2
Plasmid#46486DepositorInsertShcA (SHC1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 476-584PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-30b-3p
Plasmid#103411PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-30b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-30b-3p target (MIR30B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-myc-BioID-C
Plasmid#174737PurposeOverexpresionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-LaminB1-10
Plasmid#57966PurposeLocalization: Nuclear Envelope, Excitation: 549, Emission: 565DepositorInsertLaminB1 (LMNB1 Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Fibrillarin-7
Plasmid#58016PurposeLocalization: Nucleoli, Excitation: 555, Emission: 584DepositorInsertFibrillarin (FBL Human)
UseTagsmTagRFPExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX SH2A-SH2
Plasmid#46481DepositorInsertSH2A (SH2D4A Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 338-445PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
mOrange2-EB3-7
Plasmid#57953PurposeLocalization: MT End Binding Protein, Excitation: 549, Emission: 565DepositorInsertEB3 (MAPRE3 Human)
UseTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
TOP2A sgRNA1
Plasmid#138190Purpose3rd generation lentiviral gRNA plasmid targeting human TOP2ADepositorInsertTOP2A (TOP2A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
TOP2A sgRNA2
Plasmid#138191Purpose3rd generation lentiviral gRNA plasmid targeting human TOP2ADepositorInsertTOP2A (TOP2A Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172669PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertRNF2 pegRNA and RNF2_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
mOC-STAMP_3xFlag-Myc-CMV26
Plasmid#73577PurposeExpress mouse OC-STAMP in mammalian cellsDepositorInsertOC-STAMP (Ocstamp Mouse)
UseTags3x Flag and c-Myc tagExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only