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Addgene
Showing: 6761 - 6780 of 16025 results

  1. p663-UBC-miniTurbo-V5-VRK2_IDG-K

    Plasmid
    #158196
    Purpose
    Gateway destination clone of VRK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-VRK2 (VRK2 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    158196-plasmid-map-sequence-id-314666

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  2. pET28-6His-SUMO-Nsp14

    Plasmid
    #154956
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    154956-plasmid-map-sequence-id-307845

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  3. pET28-6His-SUMO-Nsp10

    Plasmid
    #154959
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    154959-plasmid-map-sequence-id-307848

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  7. pB6-Id1-loxP-AU1-rox-FNF-loxP-drePBD*-rox

    Plasmid
    #168770
    Purpose
    B6J Id1 AU1 tag and LSL-TagRFP-T-drePBD* targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Tags
    Expression
    Mutation
    See Depositor Comments Below
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    168770-plasmid-map-sequence-id-337553

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  9. p663-UBC-miniTurbo-V5-NEK5_IDG-K

    Plasmid
    #189876
    Purpose
    Gateway destination clone of NEK5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK5 (NEK5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    189876-plasmid-map-sequence-id-374161

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  10. p667-UBC-NEK7-V5-miniTurbo_IDG-K

    Plasmid
    #189882
    Purpose
    Gateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    NEK7 (NEK7 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    189882-plasmid-map-sequence-id-374826

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  11. p667-UBC-VRK3-V5-miniTurbo_IDG-K

    Plasmid
    #189902
    Purpose
    Gateway destination clone of VRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    VRK3 (VRK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    189902-plasmid-map-sequence-id-381809

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  13. pIA030

    Plasmid
    #200424
    Purpose
    thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.
    Depositor
    Rut Carballido-López
    Article
    Altinoglu et al bioRxiv 2023
    Insert
    LacI
    Use
    Tags
    PAmCherry
    Expression
    Bacterial
    Mutation
    Promoter
    Ppen
    Availability
    Academic Institutions and Nonprofits only
    200424-plasmid-map-sequence-id-405349

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  14. p663-UBC-miniTurbo-V5-NEK11_IDG-K

    Plasmid
    #198005
    Purpose
    Gateway destination clone of NEK11 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-NEK11 (NEK11 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    198005-plasmid-map-sequence-id-390022

    Enlarge

  15. p663-UBC-miniTurbo-V5-NEK3_IDG-K

    Plasmid
    #198006
    Purpose
    Gateway destination clone of NEK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-NEK3 (NEK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
    198006-plasmid-map-sequence-id-390032

    Enlarge

  16. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  17. pET28-eIF4E-TwStrep

    Plasmid
    #208638
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
    Depositor
    Quanfu Ma
    Insert
    human eIF4E (ENST00000450253) (EIF4E Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    208638-plasmid-map-sequence-id-415918

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  20. pEF1-puro/hpb1

    Plasmid
    #59976
    Purpose
    integrin b1 short headpiece expression (beta-I, hybrid, PSI and I-EFG1 domains) expression
    Depositor
    Timothy Springer
    Insert
    human integrin b1 headpiece (ITGB1 Human)
    Use
    Tags
    Base coiled-coil and His-tag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 6761 - 6780 of 16025 results