-
Plasmid#172126PurposeExpression of InteinC-tTAC in Cre recombinase positive cellsDepositorInsertInteinC-tTAc
UseAAVTagsExpressionMutationPromoterCaMKIIAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FRT-EBFP-tTAn
Plasmid#172127PurposeExpression of EBFP and tTA-InteinN in Flp recombinase positive cellsDepositorInsertEBFP, tTAn-InteinN
UseAAVTagsExpressionMutationPromoterEF1aAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-XL4 ASXL1 (p.R404X) 3x FLAG
Plasmid#74263PurposeCancer-associated ASXL1 truncation mutant in mammalian expression vectorDepositorInsertASXL1 (ASXL1 Human)
UseTags3X FLAGExpressionMammalianMutationp.R404X truncationPromoterCMVAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP
Plasmid#114729Purpose3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc geneDepositorInsertsgRNA M5, sgRNA M7, sgRNA M9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330 _HITI donor B6
Plasmid#172847PurposeDRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).DepositorInsertDRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 2
Plasmid#128571Purposefor cloning identified individual SPECSDepositorTypeEmpty backboneUseLentiviralTagsTagRFPExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19b-3p
Plasmid#103327PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19b-3p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Rin1-SH2
Plasmid#46474DepositorInsertRin1 (RIN1 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 54-204PromoterTacAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag
Plasmid#185683PurposeLow-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Human, Synthetic)
UseTagsHaloTagExpressionMammalianMutation(WLLI-GSGS)[359-362]PromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX4
Plasmid#183903Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PUbAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX3
Plasmid#183904Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ESR1-luciferase
Plasmid#113351Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 geneDepositorInsertESR1-enhancer (ESR1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-143-3p
Plasmid#103242PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-143-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-143-3p target (MIR143 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-Dicer
Plasmid#90013PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-DicerDepositorInsertsCBirA*-Ago2
NBirA*-Dicer
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry
Plasmid#179428PurposeDual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertTRE-cit-(nospacer)-mch
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Vav2-SH2
Plasmid#46529DepositorInsertVav2 (VAV2 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 665-774PromoterTacAvailable sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf
Plasmid#130976PurposeExpression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.DepositorInsertHook3-SNAPf (HOOK3 Human)
UseTags8His-ZZ-LTLT and SNAPfExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
LAP2-beta-actin in modified pEGFP
Plasmid#34839DepositorInsertLAP2-beta-Actin (ACTB Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCsy3-VPR-Csy4
Plasmid#153943PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy3-VPR and Csy4 in mammalian cellsDepositorInsertCsy3-VPR, Csy4
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCsy1-Csy2
Plasmid#153942PurposeExpresses type I-F P. aeruginosa cascade (PaeCascade) proteins Csy1 and Csy2 in mammalian cellsDepositorInsertCsy1, Csy2
UseAAV and CRISPRTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only