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Plasmid#217892PurposeLentiviral vector, entry vector for Lentiviral Switch-OVERDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only
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pGGC-LexACasPea
Plasmid#210539PurposeCloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module CDepositorInsertLexA::SpCas9::PEA3AT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYN2_1
Plasmid#184757PurposePlasmid for genome editing by CRISPR/Cas9DepositorInsertsCas9
sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
UseSynthetic BiologyTags2xNLSExpressionBacterial and YeastMutationPromoterPGK1 and tRNA-Phe-HDV RibozymeAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-sTagRFP-TUBA1B
Plasmid#207765PurposeDonor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD_TypeIC_Cascade_cas3_invivo
Plasmid#196400Purposeexpresses CRISPR Cascade-Cas3 Type IC proteins for in vivo assayDepositorInsertsCas5c
Cas7c
Cas8c
Cas3c
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PlmCasX-T2A-EGFP
Plasmid#188273PurposeMammalian expression, Genome editingDepositorInsertPlmCasX
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHACK(Gal4)-DONR(T2A-Cas9)
Plasmid#194768PurposeTo insert T2A-Cas9 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Cas9 transgene.DepositorInsertT2A-Cas9
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK411.RR1
Plasmid#192644PurposeExpresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpRDepositorInsertBBa_J23119-RR1
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterBBa_J23119Available sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS
Plasmid#191365PurposeOverexpression of NLS-dPbaCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPbaCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS
Plasmid#191364PurposeOverexpression of NLS-dPba5Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba5Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS
Plasmid#191363PurposeOverexpression of NLS-dPba4Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba4Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-NLS-dMisCas13b-SNAPf-NLS-3xFlag
Plasmid#191536PurposeOverexpression of NLS-dMisCas13b-SNAPf-NLS-3xFlag in human cellsDepositorInsertNLS-dMisCas13b-SNAPf-NLS-3xFlag
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS
Plasmid#191366PurposeOverexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dHgm6Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS
Plasmid#191362PurposeOverexpression of NLS-dBba2Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dBba2Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS
Plasmid#191361PurposeOverexpression of NLS-dPba3Cas13b-EGFP-NLS in human cellsDepositorInsertNLS-dPba3Cas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dMisCas13b-EGFP-NLS
Plasmid#191359PurposeOverexpression of NLS-dMisCas13b-EGFP-NLS in human cellsDepositorInsertNLS-dMisCas13b-EGFP-NLS
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
DpbdCas12e KRAB
Plasmid#188505PurposeExpresses FLAG tagged DpbdCas12e KRABDepositorInsertdCas12e
UseCRISPR and LentiviralTagsExpressionMammalianMutationD672A/E769A/D935APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT004
Plasmid#182714PurposeConstituitvely expressed CasRx crRNA cloning vector, extended 3' terminator regionDepositorInsertCasRx crRNA cloning backbone
UseCRISPRTagsExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)Available sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only