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pY036_ATP1A1_G3
Plasmid#86617PurposeExpresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1DepositorInsertATP1A1 G3 crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianMutationPromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 Q147A, M151A, I154A
Plasmid#74616PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationQ147A, M151A, I154APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 D102A, E108A
Plasmid#74618PurposeExpresses a double mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 N68A, W69A, K77A
Plasmid#74619PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22-HT/purC
Plasmid#73623PurposeProduces Escherichia coli 5-aminoimidazole-4-N-succinocarboxamide ribonucleotide synthetase (PurC), N-terminal His6 tagDepositorInsertphosphoribosylaminoimidazolesuccinocarboxamide synthase (purC Escherichia coli str. K-12 substr. MG1655)
UseTagsHis6ExpressionBacterialMutationContains multiple silent mutations (E179, G185, G…PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-R5A-K6A C11orf83-V5
Plasmid#65850PurposeMammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6ADepositorInsertMutated UQCC3 (UQCC3 Human)
UseTagsV5ExpressionMammalianMutationR5A-K6APromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-R5D-K6D C11orf83-V5
Plasmid#65851PurposeMammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6DDepositorInsertMutated UQCC3 (UQCC3 Human)
UseTagsV5ExpressionMammalianMutationR5D-K6DPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR4-*mRFP
Plasmid#47118PurposeCan be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.DepositorInsert*mRFP
UseTagsExpressionBacterialMutationNo start codonPromoterLacAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-32-Decoy.hPGK.GFP.WPRE
Plasmid#46608DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-759-Decoy.hPGK.GFP.WPRE
Plasmid#46634DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-761-Decoy.hPGK.GFP.WPRE
Plasmid#46635DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.U6.miR-192-Decoy.hPGK.GFP.WPRE
Plasmid#46597DepositorUseLentiviralTagsExpressionMutationPromoterhU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only
