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R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

Plasmid

#166020

Purpose

mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

Depositor

Dominic Esposito

Article

Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

Insert

SARS-CoV-2 RBD (S )

Use

Tags

3C-His8-SBP

Expression

Mammalian

Mutation

Promoter

CMV51

Available since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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Met1 diUb (1-152, R42W)

Plasmid

#110767

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

R42W

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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tdEos-EB3-7

Plasmid

#57610

Purpose

Localization: MT End Binding Protein, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

EB3 (MAPRE3 Human)

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pJL1-eBFP2

Plasmid

#102640

Purpose

In vitro expression of eBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

eBFP2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-mTFP1

Plasmid

#102635

Purpose

In vitro expression of mTFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Mito-DsRed1

Plasmid

#87379

Purpose

Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

Depositor

Björn Stork

Article

Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

Insert

TXN2 mitochondrial transit peptide (TXN2 Human)

Use

Retroviral

Tags

DsRed1

Expression

Mammalian

Mutation

First 60 aa only

Promoter

Retroviral 5' LTR promoter

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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sgKRAS(B)

Plasmid

#85567

Purpose

Expresses sgRNA targeting human KRAS around codon G12

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

KRAS proto-oncogene, GTPase (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
sgKRAS(A)

Plasmid

#85566

Purpose

Expresses sgRNA targeting human KRAS around codon G12

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

KRAS proto-oncogene, GTPase (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
AAV-hSyn-ASAP4e-Kv-WPRE

Plasmid

#201031

Purpose

Expresses somatically enriched ASAP4e in neurons, can be used to package AAV

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-FLEx-ASAP4e-Kv-WPRE

Plasmid

#201033

Purpose

Expresses somatically enriched ASAP4e from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4b-Kv

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-mRFP1

Plasmid

#102630

Purpose

In vitro expression of mRFP1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ASAP4b-Kv-WPRE

Plasmid

#201030

Purpose

Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4b-Kv

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-C6F/TdTomato

Plasmid

#140826

Purpose

OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)

Depositor

Hideyuki Okano

Article

Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

Inserts

OCT4 (POU5F1 Human)
SOX2 (SOX2 Human)
KLF4 (KLF4 Human)
C-MYC (MYC Human)
KLF2 (KLF2 Human)
NANOG (NANOG Human)
tdTomato

Use

Tags

Expression

Mammalian

Mutation

L104P- please see depositor comments

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

Plasmid

#201032

Purpose

Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4e-Kv

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-pro-Il1b-C-Flag

Plasmid

#75131

Purpose

Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

interleukin 1 beta (Il1b Mouse)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

(please see depositor comments below)

Promoter

CMV

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 711-1034

Plasmid

#75141

Purpose

Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 711-1034

Promoter

CMV

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 91-710

Plasmid

#75140

Purpose

Expresses N-terminal Flag-tagged amino acids 91-710 of NLRP3 (the nucleotide-binding domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 91-710

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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pTripz NEO DEST

Plasmid

#209072

Purpose

Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

Depositor

Matthew Oser

Article

Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

Type

Empty backbone

Use

Lentiviral; Gateway destination vector to be used…

Tags

3X FLAG

Expression

Mutation

Promoter

CMV

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-NLRP3 1-90

Plasmid

#75137

Purpose

Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NLR family, pyrin domain containing 3 (Nlrp3 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

fragment encoding amino acids 1-90

Promoter

CMV

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

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