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Plasmid#155318PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by VKTQR linker S135A mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
UseTagsHis tagExpressionBacterialMutationS135APromoterM13Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Halo-Tev- HSPB7(zebrafish) C49S
Plasmid#108914PurposeExpress C49S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cellsDepositorInsertHalo-TEV-HSPB7
UseTagsHalo-TEVExpressionMammalianMutationCysteine 49 to SerinePromoterSP6Available sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-XYLT2-sgRNA
Plasmid#154862PurposeLentiviral expression of Cas9 and sgRNA targeting XYLT2DepositorInsertXYLT2 sgRNA (XYLT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-FRT-TO-EGFP-RIP2_3K
Plasmid#131205Purposemammalian expression, tet RDepositorInsertRIPK2 K209/410/538R (RIPK2 Human)
UseTagsEGFPExpressionMammalianMutationK209/410/538RPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.16
Plasmid#125083PurposeExpression of ompR(1-140aa)-ccaR(130-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)140
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD263
Plasmid#125094PurposeExpression of torR(1-134aa)-ccaR(127-234aa), ompR137 chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertstorR(REC)-ccaR(DBD)137
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.25
Plasmid#125085PurposeExpression of ompR(1-110aa)-ccaR(107-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)110
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL436 HEK293site3EQR sgRNA
Plasmid#126445PurposeTo cut the human HEK293 site3 locus for integration.DepositorInsertspacer against human HEK293 site3 with NGAG PAM
UseTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.28
Plasmid#125087PurposeExpression of ompR(1-138aa)-ccaR(128-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)138
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.26
Plasmid#125086PurposeExpression of ompR(1-113aa)-ccaR(110-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsOmpR(REC)-ccaR(DBD)113
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.30
Plasmid#125089PurposeExpression of ompR(1-142aa)-ccaR(132-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)142
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationL119M- see depositor comments belowPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.18
Plasmid#125084PurposeExpression of ompR(1-150aa)-ccaR(140-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)150
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.14
Plasmid#125081PurposeExpression of ompR(1-122aa)-ccaR(119-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)122
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR40.13
Plasmid#125080PurposeExpression of ompR(1-118aa)-ccaR(115-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172DepositorInsertsompR(REC)-ccaR(DBD)118
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2001
Plasmid#105871PurposeHigh copy HRPT backbone plasmid for insertion of MiniP promotersDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKP105 (pRS416-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106461PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)
Plasmid#106470PurposeExpresses Atg42/Ybr139w with a C-terminal PA tag in yeast cellsDepositorInsertYBR139W
UseTagsPAExpressionBacterial and YeastMutationPromoterYBR139WAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
St1Cas9_LMD-9_sgRNA_pUC19
Plasmid#110627PurposeU6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).DepositorInsertEmpty St1Cas9 LMD-9 sgRNA backbone
UseCRISPR and Synthetic BiologyTagsNoneExpressionMammalianMutationPromoterhU6Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only