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Addgene
Showing: 6721 - 6740 of 16025 results

  1. pET28-Nsp14-TEV-Strep

    Plasmid
    #154953
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    Use
    Tags
    TEV-Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    154953-plasmid-map-sequence-id-307852

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  2. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

    Enlarge

  3. pET28-Strep-TEV-Nsp14

    Plasmid
    #154955
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Strep-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    Strep-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    154955-plasmid-map-sequence-id-307850

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  8. L3MBTL1 (3P8H)

    Plasmid
    #28265
    Depositor
    Cheryl Arrowsmith
    Insert
    L3MBTL1 (L3MBTL1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 200-530.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. RFXANK (3V3O)

    Plasmid
    #36905
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RFXANK (RFXANK Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    amino acid residues 90-260 (ANK repeats)
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. pMito-rxRFP1.1

    Plasmid
    #67841
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.1, mitochondrially localized
    Depositor
    Huiwang Ai
    Article
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Insert
    rxRFP1.1 and mitochondrial localization sequence
    Use
    Tags
    His Tag and mitochondrial localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    67841-plasmid-map-sequence-id-244286

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  12. pNuc-rxRFP1.1

    Plasmid
    #68131
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.1, nuclear localization
    Depositor
    Huiwang Ai
    Article
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Insert
    rxRFP1.1 and nuclear localization sequence
    Use
    Tags
    His Tag and nuclear localization sequence
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only

  13. pJK625

    Plasmid
    #72541
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit with N-terminal His6 tag (H6AaPurS); glutaminase subunit (AaPurQ); large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    synthetic gene
    Promoter
    T7 and n/a
    Availability
    Academic Institutions and Nonprofits only
    72541-plasmid-map-sequence-id-132634

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  16. pFRT-TODestFLAGHA_HuC

    Plasmid
    #65756
    Purpose
    expresses FLAGHA-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Availability
    Academic Institutions and Nonprofits only
    65756-plasmid-map-sequence-id-111826

    Enlarge

  17. pFRT-DestFLAGHA_HuC

    Plasmid
    #65759
    Purpose
    constitutively expresses FLAG-HA-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Availability
    Academic Institutions and Nonprofits only

  18. pFRT-TODestGFP_HuC

    Plasmid
    #65762
    Purpose
    expresses GFP-tagged HuC in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Availability
    Academic Institutions and Nonprofits only
    65762-plasmid-map-sequence-id-111834

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Showing: 6721 - 6740 of 16025 results