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Plasmid#81183PurposeExpresses Desulfovibrio vulgaris Cas8c in E. coli with TEV-cleavable N-terminal His-MBP tagDepositorInsertCas8c
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialMutationPromoterT7Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUb LwaCas13a + LwaCas13a Guide RNA
Plasmid#176307PurposeExpresses LwaCas13a and its associated guide RNA in Drosophila cellsDepositorInsertLwaCas13a
UseTagsHA tagExpressionInsectMutationE656G, I1060V, D1076G- please see depositor comme…PromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MLX
Plasmid#86277PurposeDonor vector for 3' FLAG tag of human MLXDepositorInsertMLX homology arms (MLX Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEJS507-pCDest2-noAcr-mTagBFP2-IRES
Plasmid#85748PurposeExpresses mTagBFP2 only; used as a controlDepositorInsertmTagBFP2
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAflaB
Plasmid#149642Purposeall-in-one CRISPRi vector for targeting B. burgdorferi flaBDepositorInsertdCas9, lacI, sgRNAflaB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK526
Plasmid#155387PurposePphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
PphlF-TA4 in dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK413
Plasmid#155372PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM355
Plasmid#173175Purpose(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoterDepositorInsertgRNA-12xMBS backbone
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM215A
Plasmid#173217PurposeExpresses ScFV-GCN4-sFGFP10M2-GB1DepositorInsertScFV-GCN4-sfGFP10M2-GB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pM214
Plasmid#173216PurposeExpresses MCP-mCherry-GFP11-GB1DepositorInsertMCP-mCherry-GFP11-GB1
UseCRISPR and LentiviralTagsHA and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNAmreB
Plasmid#149653Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AmCas12b-2AeGFP
Plasmid#121947PurposeMammalian expression, Genome editingDepositorInsertAmCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF204
Plasmid#121656PurposeU6-sgRNA EFS-Cas9-wt-P2A-PuroDepositorInsertCas9
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA
Plasmid#133344Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_cpaA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZGPAT
Plasmid#86276PurposeDonor vector for 3' FLAG tag of human ZGPATDepositorInsertZGPAT homology arms (ZGPAT Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pM117: VEGFA array presgRNA
Plasmid#166869PurposeU6-driven expression of human VEGFA targeting array presgRNA compatible with CasRx.DepositorInsertHuman VEGFA targeting array presgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK429
Plasmid#155376PurposePphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]DepositorInsertsdCas9 (bacteria)
PA4-mVenus
PphlF_A4NT in dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_ZBTB7B
Plasmid#135730PurposeDonor vector for 3' FLAG tag of human ZBTB7BDepositorInsertZBTB7B Homology arms (ZBTB7B Human)
UseCRISPRTags3XFLAG-P2A-NeoRExpressionMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
dSp-VP64-p65(150-261)-RTA(125-190)
Plasmid#99674PurposeExpresses dSp Cas9 fused to combination of truncated activation domainsDepositorArticleInsertdCas9
UseTagsVP64-P65(151-261)-RTA(125-190)ExpressionMammalianMutationdead Cas9PromoterAvailable sinceAug. 20, 2018AvailabilityAcademic Institutions and Nonprofits only