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Wnt6 Non-STOP

Plasmid

#35895

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation G (567) --> A

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt7B Non-STOP

Plasmid

#35897

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7B (Wnt7b Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt8B Non-STOP

Plasmid

#35899

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt9A Non-STOP

Plasmid

#35900

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9A (WNT9A Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation A (543) --> G

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBacPAK8-VHL(1-155)

Plasmid

#29500

Depositor

Ronald Conaway, Joan Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Use

Tags

Expression

Insect

Mutation

VHL(1-155)only

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pAJ03

Plasmid

#14910

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Ss era-1 3' UTR

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ04

Plasmid

#14911

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Ss era-1 3' UTR

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pJCNM(del22-68)

Plasmid

#1095

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

amino acids 20 - 68 DELETED from NM domain of SUP…

Promoter

Available since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pCellFree_G06_ZIKV NS2B

Plasmid

#145354

Purpose

Cell-free expression vector for Zika virus NS2B protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2B

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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PG13-mCherry

Plasmid

#195291

Purpose

mCherry reporter containing 13 copies of the p53-binding consensus sequence

Depositor

Hideyuki Oguro

Article

Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-Bcl-XL

Plasmid

#46972

Purpose

lentiviral expression of Bcl-XL

Depositor

Jialiang Wang

Article

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

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lenti-EF1a-dCas9-VPR-Puro

Plasmid

#99373

Purpose

3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

(Sp)dCas9-VPR-P2A-Puro

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Plasmid

#207860

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Rosa26-twinPE-attB

Use

AAV

Tags

Expression

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Crimson

Plasmid

#70683

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.

Depositor

Daniel Bauer

Article

Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

Inserts

S. pyogenes sgRNA cassette
Crimson Reporter

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Ef1-a and hU6

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Plasmid

#207863

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Use

AAV

Tags

Expression

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Tags

Expression

Mutation

See Manuscript

Promoter

Cbh

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_5-HT1.0

Plasmid

#140552

Purpose

Expresses the 5-HT sensor GRAB_5-HT1.0 in neurons

Depositor

Yulong Li

Article

Wan et al Nat Neurosci. 2021 Apr 5. pii: 10.1038/s

Has Service

AAV9

Insert

5-HT sensor GRAB_5-HT1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHTN_HaloTag_KIF5B

Plasmid

#213404

Purpose

Full-length human kinesin-1 with HaloTag at the N-terminus

Depositor

Jonas Ries

Article

Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

Insert

HaloTag-KIF5B (KIF5B Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDRM166 LKB (Luciferase mKate2 Blast)

Plasmid

#183502

Purpose

Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

Firefly Luciferase
mKate2
BSD

Use

Lentiviral and Luciferase

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Mutation

Promoter

MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PSMA1-PSMA2-PSMA3-PSMA4

Plasmid

#217893

Purpose

Insect cell expression of human 20S CP proteasome subunit alpha type-1 (PSMA1), alpha type-2 (PSMA2), alpha type-3 (PSMA3), and alpha type-4 (PSMA4)

Depositor

Brenda Schulman

Article

Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

Inserts

PSMA1 (PSMA1 Human)
PSMA2 (PSMA2 Human)
PSMA3 (PSMA3 Human)
PSMA4 (PSMA4 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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