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Showing: 10051 - 10080 of 148587 results

  1. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-354311

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  2. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid
    #194328
    Purpose
    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness
    Depositor
    Alexander McLellan
    Article
    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:
    Insert
    Luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Tet-responsive promotor related mutations: G72V i…
    Promoter
    Tight tet-responsive promotor
    Available since
    Jan. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    194328-plasmid-map-sequence-id-383152

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  3. pCAT003

    Plasmid
    #171628
    Purpose
    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-TRAC
    mNeon
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and EF1-a
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171628-plasmid-map-sequence-id-336485

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  4. pT3-EF1A-MYC-IRES-lucOS

    Plasmid
    #129776
    Purpose
    Transposon based vector that expresses human MYC followed by IRES and firefly luciferase and the antigens SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), and OVA323-339
    Depositor
    Amaia Lujambio
    Article
    Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82
    Insert
    human MYC (MYC Human)
    Use
    Tags
    OS: SIYRYYGL (SIY), SIINFEKL (SIN, OVA257-264), a…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    129776-plasmid-map-sequence-id-263368

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  19. lentiGuide-Crimson

    Plasmid
    #70683
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.
    Depositor
    Daniel Bauer
    Article
    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature
    Inserts
    S. pyogenes sgRNA cassette
    Crimson Reporter
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1-a and hU6
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    70683-plasmid-map-sequence-id-217910

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  21. pLEX307-FURIN-G418

    Plasmid
    #158462
    Purpose
    Constitutive lentiviral expression of human FURIN.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    FURIN (FURIN Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158462-plasmid-map-sequence-id-308175

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  24. enOgeuIscB with ωRNA-v2

    Plasmid
    #220939
    Purpose
    CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v2 scaffold with BsmBI for guide cloing
    Depositor
    Weixin Tang
    Article
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Inserts
    CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
    OgeuIscB-ωRNA-v2 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220939-plasmid-map-sequence-id-443121

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  28. pBV58

    Plasmid
    #204603
    Purpose
    ERG11-3X Minidegron-5X FLAG-HIS3MX6
    Depositor
    Scott Moye-Rowley
    Insert
    Candida glabrata ERG11 (ERG11 )
    Use
    Tags
    auxin-inducible degron
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204603-plasmid-map-sequence-id-403443

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Showing: 10051 - 10080 of 148587 results