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mEGFP-HRas S17N
Plasmid#18665DepositorInsertH-Ras (HRAS Human)
UseTagsmonomeric EGFPExpressionMammalianMutationS17N mutation in H-RasPromoterAvailable sinceJuly 2, 2008AvailabilityAcademic Institutions and Nonprofits only -
mH2A1.1-CT-GFP
Plasmid#45167DepositorInsertH2Afy (MACROH2A1 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG303-Gal-PR50
Plasmid#84905Purposeinduce expression of dipeptide repeat PR in yeastDepositorInsertPR50 (C9orf72 Synthetic)
UseTagsFLAG and MycExpressionYeastMutationPromoterGAL1Available sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ConFoff-TVA(mChr)-W3SL
Plasmid#218756PurposeProvides NOT intersectional (Cre NOT Flp) expression of TVA-mCherryDepositorInsertTVA-mCherry
UseAAVTagsmCherryExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-CoffFon-TVA(mChr)-W3SL
Plasmid#218755PurposeProvides NOT intersectional (Flp NOT Cre) expression of TVA-mCherryDepositorInsertTVA-mCherry
UseAAVTagsmCherryExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV5.1-SARS-CoV-2 Nsp3
Plasmid#204205PurposeMammalian expression of SARS-CoV-2 Nsp3DepositorInsertSARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
UseTagsFLAGx3ExpressionMammalianMutationnone, from obtained clonePromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3G-p300-dCas9-P2A-mCherry
Plasmid#138456Purpose2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherryDepositorInsertp300 core
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K29
Plasmid#22903DepositorInsertUbiquitin C (UBC Human)
UseTagsHAExpressionMammalianMutationK29 only, all other lysines mutated to argininesPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
AAV.hSyn.GLPLight-ctr
Plasmid#187468PurposeExpresses GLPLight-ctr in mammalian cellsDepositorInsertGLPLight-ctr
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GH
Plasmid#60498PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
miniCMV-tdTomato-tDeg
Plasmid#217521PurposeExpresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoterDepositorInserttdTomato-tDeg fluorogenic protein
UseTagsExpressionMammalianMutationPromoterminiCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
EC70195
Plasmid#209456PurposeCloning protospacers for Cas9 editingDepositorInsertCas9 sgRNA
UseCRISPRTagsExpressionMutationPromoterTaU3Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSV2G_PflaB-mCherryBb
Plasmid#118230PurposeGentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mCherryDepositorInsertflagellin promoter driven mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FEN1
Plasmid#215129PurposeExpresses mCherry-FEN1 in mammalian cells from a retroviral vector.DepositorInsertFEN1 (FEN1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-p50-ligase
Plasmid#87742PurposeIPTG-inducible expression of p50-ligase fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and human p50/NF-kB (amino acids 40-366)ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBA559
Plasmid#186235PurposepTet-LbuCas13a crRNA entry vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330.puro
Plasmid#110403PurposeCRISPR/Cas9 vector with sgRNA expression and puromycin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-BCL6-t2A-BCL2
Plasmid#135305PurposeOverexpression of multiple human genes (BCL2 and BCL6)DepositorUseRetroviralTagsExpressionMutationPromoterLTRAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only
