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Plasmid#25701DepositorInsertAxin 2 promoter (AXIN2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
UseTagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAcCas12nHS_RV6_PT1_empty
Plasmid#203810PurposePlasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.DepositorInsertAcCas12nHS_RV6_PT1
UseCRISPRTagsExpressionMutationPromoterCMVAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP-Stim1-ΔCTD
Plasmid#186621PurposeEncodes Stim1 with C-terminal domain deletion fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationDeletion of C-terminal domain 601 to 685PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-2P
Plasmid#203748PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27AC30A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27A, C30APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEBB COMMD9 Flag
Plasmid#74901PurposeExpress COMMD9 in mammalian cellsDepositorInsertCOMMD9 (COMMD9 Human)
UseTagsFlagExpressionMammalianMutationPromoterEF-1alpha promoterAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-Cre
Plasmid#47117PurposeCan be used to in vitro transcribe Cre recombinase.DepositorInsertCre
UseTagsExpressionMutationPromoterLacAvailable sinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
Kif1A(1-489)-DHFR-myc
Plasmid#117833PurposeConstitutively active Kinesin-3 motor tagged with DHFR and mycDepositorInsertKif1A (1-489) (Kif1a Mouse)
UseTagsDHFR and mycExpressionMutationonly aa 1-489 (motor domain)PromoterCMVAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
plVX-EF1a-FLAG-hMETTL3-IRES-ZsGreen
Plasmid#207123PurposeLentiviral vector to express human METTL3DepositorInserthMETTL3 (METTL3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG
Plasmid#154777Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
UseTagsExpressionMammalianMutation102TAA 150TAG in GFP reporter, hybrid PylT with …PromoterEF1Available sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
LLP109 pEF1a-gRNA-GFP
Plasmid#100935PurposeGFP, puromycin and gRNA scaffold driven by EF1a promoterDepositorInsertgRNA scaffold
UseGrna scaffoldTagsExpressionMammalianMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST
Plasmid#34922DepositorInsertp53 DNA-binding (Res 1-393) (TP53 Human)
UseTagsExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Actin-7
Plasmid#58001PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorInsertActin (ACTB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-EF1a-Flex-CA-AMPK-H150R-T2A-mCherry
Plasmid#83490Purposecre-dependent CA-AMPK under EF1a promoterDepositorInsertCA-AMPK-2A-mCherry
UseAAV and Cre/LoxTagsExpressionMammalianMutationH150RPromoterAvailable sinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Accessory plasmid 1
Plasmid#128569Purposefor mixing with SPECS library to produce lentivirusDepositorInsertECFP
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX601-AAV-CMV-NLS-ISTfu1-NLS-3xHA-bGHpA;U6-BbsI-reRNA
Plasmid#205319PurposeAll-in-one AAV vector expressing ISTfu1 TnpB and its reRNADepositorInserthumanized ISTfu1 TnpB
UseAAVTags3 x HAExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-191-5p
Plasmid#103300PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-191-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-191-5p target (MIR191 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF3-Flag-Cbir-Ago2_Myc-Nbir-TNRC6C
Plasmid#90014PurposeSplit-BioID plasmid: Expresses N-terminally tagged CBir[257-321]-Ago2 and N-terminally tagged NBir[2-256]-TNRC6CDepositorInsertsCBirA*-Ago2
NBirA*-TNRC6C
UseRetroviral; Flp/frtTagsFLAG and MycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-CaMKIIa (T305D/T306D)
Plasmid#127611PurposeFluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)DepositorInsertcalcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)
UseTagsmEGFPExpressionMammalianMutationchanged Threonine 305/306 to AlaninePromoterpCAGAvailable sinceJan. 25, 2020AvailabilityAcademic Institutions and Nonprofits only