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pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

Plasmid

#112214

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3A-L-dcas9 (DNMT3L, DNMT3A Human)

Use

CRISPR

Tags

mCherry

Expression

Mutation

D10A,H840A,N863A

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

Plasmid

#112215

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)

Use

CRISPR

Tags

mCherry

Expression

Mutation

D10A,H840A,N863A,C710S

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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BRI1 BRI1_pECIA2

Plasmid

#115086

Purpose

Bait vector BRI1 BRI1_pECIA2 should be used with prey vector BRI1 BRI1_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT4G39400 (BRI1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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RPK2 X042_pECIA2

Plasmid

#115135

Purpose

Bait vector RPK2 X042_pECIA2 should be used with prey vector RPK2 X042_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT3G02130 (RPK2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Rb379-928/C706F-pGex2TK

Plasmid

#21750

Depositor

William Kaelin

Article

Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

Insert

Rb (RB1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

deletion mutant ORF contains aa 379-928 of wt Rb …

Promoter

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

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HACE1_L (OZ565)

Plasmid

#33379

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting HACE1 (LOC572430 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
HACE1_R (OZ566)

Plasmid

#33380

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting HACE1 (LOC572430 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
col6a2_R (OZ594)

Plasmid

#35234

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting col6a2 (col6a2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
col6a2_L (OZ593)

Plasmid

#35233

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting col6a2 (col6a2 Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pnCS_DSep2_Peanut-TEV-Strep

Plasmid

#174495

Purpose

bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

Sep2 (Sep2 Fly)
peanut (pnut Fly)

Use

Tags

TEV-Strep

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Venus1-WTSTAT3

Plasmid

#123164

Purpose

Expresses wild type STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

none

Promoter

pCMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMx-MGT

Plasmid

#111810

Purpose

Retroviral expression vector for direct cardiac reprogramming

Depositor

Li Qian

Article

Wang et al Circ Res. 2015 Jan 16;116(2):237-44. doi

Insert

Mef2c, Tbx5 and Gata4 (Gata4, Mef2c, Tbx5 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

viral LTR

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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Venus1-S727A-STAT3

Plasmid

#123174

Purpose

Expresses S727A STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

S727A substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-Y705F-STAT3

Plasmid

#123172

Purpose

Expresses Y705F STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

Y705F substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus2-WTSTAT3

Plasmid

#123165

Purpose

Expresses wild type STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

none

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K685R-STAT3

Plasmid

#123170

Purpose

Expresses K658R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K685R substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus2-Y705F-STAT3

Plasmid

#123173

Purpose

Expresses Y705F STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

Y705F substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus2-S727A-STAT3

Plasmid

#123175

Purpose

Expresses S727A STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

S727A substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus1-K49R-STAT3

Plasmid

#123166

Purpose

Expresses K49R STAT3 fused to the 1-157 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)

Use

Tags

Venus1 (aa 1-158) for bimolecular fluorescence co…

Expression

Mammalian

Mutation

K49R substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Venus2-K49R-STAT3

Plasmid

#123167

Purpose

Expresses K49R STAT3 fused to the 158-238 fragment of Venus in mammalian cells

Depositor

Federico Herrera

Article

Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.

Insert

STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)

Use

Tags

Venus2 (aa 158-238) for bimolecular fluorescence …

Expression

Mammalian

Mutation

K49R substitution

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge