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macroH2A1.2-GFP (pc2189)

Plasmid

#223708

Purpose

Expression of macroH2A1.2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A1.2-GFP (Macroh2a1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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macroH2A2-GFP (pc2191)

Plasmid

#223709

Purpose

Expression of macroH2A2 tagged N-terminal to GFP

Depositor

Cristina Cardoso

Article

Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

Insert

macroH2A2-GFP (MACROH2A2, H2AFY2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-CC

Plasmid

#225476

Purpose

Express mEGFP-GAPDH-CC fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with CC2 domain of ROCK2 (GAPDH Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_SRGAP2A_SH3(W765A)_GFP

Plasmid

#222616

Purpose

Expresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.

Depositor

Franck Polleux

Article

Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

Insert

SRGAP2 (SRGAP2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Point mutation W765A (SH3 domain)

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.neo GFP shScramble

Plasmid

#220357

Purpose

Retroviral expression vector for an shScramble

Depositor

David Kashatus

Article

Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

Insert

shScramble

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

H1

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pSuperior.retro.neo GFP shDNM1L

Plasmid

#220356

Purpose

Retroviral expression vector for an shDNM1L

Depositor

David Kashatus

Article

Sweatt et al Mol Syst Biol. 2024 Sep 27. doi: 10.1038

Insert

shDNM1L (DNM1L Human)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

H1

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-BG42-GFP

Plasmid

#220202

Purpose

mini-Tn7 GFP expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG42

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-BG35-GFP

Plasmid

#220201

Purpose

mini-Tn7 GFP expression vector - pBG35 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

monomeric superfolder green fluorescent protein

Use

CRISPR

Tags

Expression

Bacterial

Mutation

monomeric superfolder green fluorescent protein

Promoter

pBG35

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*

Plasmid

#195220

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

H477/R479/S481>AAA

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL S481A

Plasmid

#195223

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

S481A

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL S481D

Plasmid

#195224

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphomimetic mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Use

Tags

Expression

Mammalian

Mutation

S481D

Promoter

Available since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAcT2-msGFP2

Plasmid

#218965

Purpose

Expresses full length GalNAcT2-msGFP2 mammalian cells

Depositor

Benjamin Glick

Insert

GalNAcT2-msGFP2 (GALNT2 Human)

Use

Tags

msGFP2

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rap1A(WT)

Plasmid

#224276

Purpose

Cell transfection and expression of Rap1A

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-RalA(WT)

Plasmid

#224274

Purpose

Cell transfection and expression of RalA

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

RalA (RALA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPSt_35Spr-GFP-t35S

Plasmid

#203592

Purpose

Fully assembled Plant STARR-seq plasmid with the 35S terminator.

Depositor

Stan Fields

Article

Gorjifard et al Nat Commun. 2024 Jul 12;15(1):5868. doi:

Inserts

CaMV 35S promoter + Zm00001d041672 5'UTR
eGFP
CaMV 35S terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pIND4-GFP-MmXYRS

Plasmid

#222745

Purpose

Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker

Depositor

Steven Boxer

Inserts

sfGFP
MmXYRS
Mm Pyl tRNA

Use

Tags

6xHis

Expression

Mutation

Contains the following substitutions: V346A, W348…

Promoter

rrnB

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-VCF1 N167A

Plasmid

#223019

Purpose

Expression of GFP-tagged VCF1 (p97 binding-deficient N167A mutant) in mammalian cells.

Depositor

Niels Mailand

Article

Mirsanaye et al Nat Commun. 2024 Mar 19;15(1):2459. doi:

Insert

VCF1 (VCF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

N167A

Promoter

CMV/TetO2

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(1-157)

Plasmid

#220743

Purpose

GAL4-sfGFP(1-157) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(1-157)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-GFP-SNX9

Plasmid

#170551

Purpose

To monitor the turnover of SNX9 in lysosomes

Depositor

Christina Towers

Article

Towers et al Dev Cell. 2021 Jul 26;56(14):2029-2042.e

Insert

SNX9 (SNX9 Human)

Use

Retroviral

Tags

SNX9 in tandem with mCherry and GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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