-
Plasmid#103889PurposeProtein expression and purification of Multiple PDZ protein PDZ5 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_6
Plasmid#103890PurposeProtein expression and purification of Multiple PDZ protein PDZ6 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_8
Plasmid#103892PurposeProtein expression and purification of Multiple PDZ protein PDZ8 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_9
Plasmid#103893PurposeProtein expression and purification of Multiple PDZ protein PDZ9 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
MPDZ_PDZ_12
Plasmid#103896PurposeProtein expression and purification of Multiple PDZ protein PDZ12 domainDepositorInsertMPDZ (MPDZ Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+R3(R112C/T180C)C
Plasmid#108194PurposeExpresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPdR-PCNA3(R112C/T180C)-P450cam fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b+3(R112C/T180C)C
Plasmid#108193PurposeExpresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coliDepositorInsertPCNA3(R112C/T180C)-P450cam fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3)PromoterT7 promoterAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+2(L171C)X
Plasmid#108192PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPCNA2(L171C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)X
Plasmid#108191PurposeExpresses PdR-PCNA2(L171C)-PdX fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationL171C (PCNA2); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+1(G108C)X
Plasmid#108189PurposeExpresses PCNA1(G108C)-PdX fusion protein in E. coliDepositorInsertPCNA1(G108C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationG108C (PCNA1); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ127B
Plasmid#99883PurposeExpress two TALE-VP64 activator linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence under 2x35S promoterDepositorInsertTALE-VP64-T2A-TALE-VP64
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
UseTagsFLAGExpressionInsectMutationPromoterhsp70Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-76
Plasmid#92288PurposePlasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cellsDepositorInsertdGW182 CED (gw Fly)
UseTagsNHAExpressionInsectMutationdGW182 CED: aa 940-1215PromoterAc5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-WNT1
Plasmid#79499PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT1 CDSDepositorInsertWNT1 (WNT1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-DXMT
Plasmid#79822PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRN168-DXMT
Plasmid#79821PurposeDsREDmonomer-based BiFC vector for plants (Positive control: DXMT)DepositorInsertCaDXMT1
UseReporter plasmidTagsRN168/DsREDmonomer (1–168)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBRC169-MXMT
Plasmid#79820PurposeDsREDmonomer-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsRC169/DsREDmonomer (169–225)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode81
Plasmid#83758Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode81
UseRetroviralTagsExpressionMutationdeletion of T in starting ATG of GFP (please see …PromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode71
Plasmid#83748Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode71
UseRetroviralTagsExpressionMutationdeletion of AA5-233 in GFP (please see depositor …PromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only