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Plasmid#227718PurposePlasmid contains GFP H2B SL2, with some nlp-51 homology sequence after, Amp ResistantDepositorInsertGFP H2B SL2 (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A GFP H2B
Plasmid#227722PurposePlasmid contains T2A GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsert(nlp-51 homology) T2A 3xNLS GFP (nlp-51 Nematode)
UseCloning vectorTagsT2A 3xNLS GFPExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SL2 GFP H2B
Plasmid#227717PurposePlasmid contains SL2 GFP H2B, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertSL2 GFP H2B (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
T2A 3xNLS GFP
Plasmid#227721PurposePlasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp ResistantDepositorInsertT2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
P30.pA.NGFR-mSrtA.mCMV.hPGK.GFP.wpre
Plasmid#226728Purposebackbone vector, GFP expressionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-T7-VHH-GFPminimizer
Plasmid#202488PurposeExpresses the nanobody GFP minimizer with T7 inductionDepositorInsertnanobody GFP minimizer
UseTags6XHisExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Amp-T7-GFPuv
Plasmid#202463PurposeExpresses GFPuv with T7 inductionDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFP-SKL
Plasmid#183727PurposeFor exrpression of GFP targeted to peroxisomesDepositorInsertEGFP
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)ExpressionMutationPromoterUbiCAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-EGFP-nonstop
Plasmid#226406PurposeExpresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.DepositorInsertEGFP without stop codon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-5.0dand5:EGFP
Plasmid#223327PurposeExpresses EGFP in Kupffer's vesicle cells in zebrafish embryosDepositorInsert5.0kb dand5 promoter (dand5 Zebrafish)
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2142 MTK3-sfGFP
Plasmid#226661PurposeMTK3 part containing the coding sequence of sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mGFP
Plasmid#212197PurposeFluorescent reporter for cytoplasmic aggregatesDepositorInsertCarboxypeptidase Y variant (PRC1 Budding Yeast)
UseTagsmEGFP (monomeric version EGFP, A206K mutation)ExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterCUP1Available sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 1.0_crEGFP
Plasmid#224790PurposeLPUtopia matching RMCE donor plasmid with MONARCH 1.0 circuit with crRNA targeting EGFP. Use BlastR for positive and HSV-TK for negative selectionDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUt-MONARCH 2.0_crEGFP
Plasmid#224793PurposeLPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.DepositorInsertscrEGFP
Triplex stablier
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMV-d2iAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3401 BtoApegRNA-sfGFP
Plasmid#226667PurposePiggyBac cargo vector encoding B to A pegRNA marked with sfGFPDepositorInsertBtoA pegRNA
UseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-FLuc0-GFPOPT
Plasmid#210594PurposeExpresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-nonoptimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUB_SM-Fluc100-GFPOPT
Plasmid#210596PurposeExpresses SM(FLAG)-optimal Firefly luciferase-optimal GFPDepositorInsertSpaghetti monster (FLAG)-optimal FLuc-Optimal GFP
UseLuciferaseTagsSpaghetti monster (FLAG)ExpressionMammalianMutationPromoterUBC humanAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSEED-LoxP-sfGFP:ALFA
Plasmid#219660PurposePermits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.DepositorInsertsfGFP:ALFA SEED Cassette (LoxP)
UseTagsExpressionMutationPromoterAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.mEGFP-CBX8
Plasmid#224710PurposeExpresses N-terminal hexahistidine-tagged human CBX8 in insect cells, with a monomeric variant EGFP tag.DepositorInsertCBX8 (CBX8 Human)
UseTagsmEGFPExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
macroH2A1.1-GFP (pc2188)
Plasmid#223707PurposeExpression of macroH2A1.1 tagged N-terminal to GFPDepositorInsertmacroH2A1.1-GFP (Macroh2a1 Rat)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only