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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIN- myc ULK1 K46I

Plasmid

#27627

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Retroviral

Tags

myc

Expression

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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pEF086 MBP-CREB 127-135-His

Plasmid

#154075

Purpose

Expresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3 UQCC2(5’UTR)-FF

Plasmid

#85487

Purpose

Firefly luciferase under the control of UQCC2 5'UTR

Depositor

Ivan Topisirovic

Article

Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

Insert

5'UTR of UQCC2 (C6orf125 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-CHD5-Y619E-FRAG

Plasmid

#68871

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa) with Y619 mutation

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa, Y619E

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
fox1 (a2bp1l)_R (OZ520)

Plasmid

#27195

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
fox1 (a2bp1l)_L (OZ519)

Plasmid

#27194

Depositor

Keith Joung

Article

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting fox1 (a2bp1l) (rbfox1l Zebrafish)

Use

Zebrafish targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pBabe-Flag-SKP1-T131A

Plasmid

#213734

Purpose

Expresses human SKP1 T131A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131A (SKP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-113-117-5A

Plasmid

#213643

Purpose

Expresses human SKP1-113-117-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-113-117-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-138-142-5A

Plasmid

#213645

Purpose

Expresses human SKP1-138-142-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-138-142-5A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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