Addgene

We narrowed to 13,073 results for: ung

Showing: 6641 - 6660 of 13073 results

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pED14xS-WT

Plasmid

#134804

Purpose

IPTG inducible expression of WT S tRNA scaffold tRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFGL815

Plasmid

#52322

Purpose

A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated transformation). There is a 69-bp MCS (multiple cloning site) which contains 12 unique restriction sites.

Depositor

Naweed Naqvi

Article

Yang et al Fungal Genet Biol. 2014 Apr 24. pii: S10

Type

Empty backbone

Use

Agrobacterium

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pIF4

Plasmid

#196069

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU6 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Mutation

Promoter

MoRP27 promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pIF3

Plasmid

#196068

Purpose

Expresses dCas9-Mxi1 with MoRP27 promoter and express gRNA with MoU3 promtoer in M. oryzae

Depositor

Kabin Xie

Article

Zhang et al mSphere. 2023 Feb 21;8(1):e0059422. doi:

Insert

dCas9-Mxi1

Use

CRISPR

Tags

Expression

Mutation

Promoter

MoRP27 promoter

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Puro.let-7g.Cre

Plasmid

#17409

Purpose

Depositor

Tyler Jacks

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Feb 28. (

Insert

Let-7g

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only

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pED14xS-11

Plasmid

#134803

Purpose

IPTG inducible expression of S-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Wildtype S tRNA (decodes TCG)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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57F03_LexA-GAD

Plasmid

#83256

Purpose

Expresses LexA-GAD under the control of 57F03 enhancer

Depositor

Leslie Vosshall

Article

Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

Insert

57F03 enhancer

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Sept. 26, 2016

Availability

Academic Institutions and Nonprofits only

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SK-YFP-SPNM-B

Plasmid

#48661

Purpose

Bacterial SP and NM repression YFP reporter: protospacer B

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

SP/NM prototspacer B/PAM with reporter EYFP

Use

CRISPR

Tags

Expression

Mutation

Promoter

lambda pR promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-Citrine (525)

Plasmid

#70718

Purpose

Expression vector for Citrine purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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DS-ST1casN-

Plasmid

#48659

Purpose

Bacterial nuclease-null ST1 Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
ST1 tracrRNA

Use

CRISPR

Tags

Expression

Mutation

D to A, DH to AA, N to A

Promoter

proC

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pED14xY-3

Plasmid

#134808

Purpose

IPTG inducible expression of Y-TAGA-Evo1 qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

Y-TAGA-Evo1 qtRNA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGT-S2

Plasmid

#122583

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAM2314-RbcS-mNG

Plasmid

#165582

Purpose

Cyanobacterial carboxysome reporter with mNeonGreen driven by native rbcS promoter

Depositor

Daniel Ducat

Article

Sakkos et al ACS Synth Biol. 2021 Jul 16;10(7):1667-1

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Prbcs

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pC2HB

Plasmid

#63734

Purpose

bait plasmid for an adapted two-hybrid system for C. albicans

Depositor

Patrick Van Dijck

Article

Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

Type

Empty backbone

Use

C. albicans bait plasmid

Tags

HA, LexA, and NLS

Expression

Mutation

Promoter

Met3

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Tags

Expression

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pGT-B2

Plasmid

#122580

Purpose

MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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PM-SP!TB

Plasmid

#48650

Purpose

Bacterial SP crRNA expression: targets SP to protospacer B (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

Bacterial SP crRNA to prototspacer B

Use

CRISPR

Tags

Expression

Mutation

Promoter

J23100 promoter

Available since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pC2HP

Plasmid

#63735

Purpose

prey plasmid for an adapted two-hybrid system for C. albicans

Depositor

Patrick Van Dijck

Article

Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

Type

Empty backbone

Use

C. albicans prey plasmid

Tags

FLAG, NLS, and VP domain

Expression

Mutation

Promoter

Met3

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pFosill-2

Plasmid

#89696

Purpose

to make Fosmids that can be converted into Illumina sequencable libraries

Depositor

Andreas Gnirke

Article

Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pThpR

Plasmid

#190613

Purpose

Expresses GFP in response to tetrahydropapaverine in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

THP4.1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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