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pDONR223_TRAF3_p.D463N

Plasmid

#81460

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

K5R; D463N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KIR3DL2_p.R96Q

Plasmid

#81444

Purpose

Gateway Donor vector containing KIR3DL2 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KIR3DL2 (KIR3DL2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R96Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKR1B1_p.F252L

Plasmid

#82743

Purpose

Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

Depositor

Matthew Meyerson, Jesse Boehm, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AKR1B1 (AKR1B1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

F252L

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-SI-112

Plasmid

#131127

Purpose

C-to-T base editing reporter

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Mutant EGFP

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ256

Plasmid

#124312

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A-PmCDA1-UGI

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2-N

Plasmid

#128308

Purpose

Express hNABP2 N-terminus (1–138 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP2 N terminal fragment (OB fold) (NABP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (1–138 aa)

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ255

Plasmid

#124310

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A-UGI

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP1-N

Plasmid

#128310

Purpose

Express hNABP1 N-terminus (1–141 aa) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

hNABP1 N-terminal domain (OB fold, 1–141 aa) (NABP1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

N-terminal domain (OB fold, 1–140 aa)

Promoter

T7

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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TRIPZ-bioGEF-UBnc-PURO

Plasmid

#208044

Purpose

Enables inducible expression of low-affinity Avitag-UBnc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

UBnc (UBC Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Expression

Mutation

L73P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192241

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mClover-NeoR (EIF3F Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192236

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192237

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192238

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_NOTCH1_ICN

Plasmid

#82087

Purpose

Gateway Donor vector containing NOTCH1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NOTCH1 (NOTCH1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

ICN (AA1761-2556 present)

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_GSK3B_WT

Plasmid

#82126

Purpose

Gateway Donor vector containing GSK3B , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

GSK3B (GSK3B Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MEF2C_WT

Plasmid

#81735

Purpose

Gateway Donor vector containing MEF2C , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MEF2C (MEF2C Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RELA_WT

Plasmid

#82160

Purpose

Gateway Donor vector containing RELA , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RELA (RELA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_JMJD5_p.A230T

Plasmid

#81429

Purpose

Gateway Donor vector containing JMJD5 ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

JMJD5 (KDM8 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

A230T

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TCF4_WT

Plasmid

#82166

Purpose

Gateway Donor vector containing TCF4 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TCF4 (TCF4 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-dnMCAK

Plasmid

#208650

Purpose

Mammalian expression plasmid expressing eGFP-dnMCAK driven by CMV promoter

Depositor

Samuel Bakhoum

Article

Li et al Nature. 2023 Aug;620(7976):1080-1088. do

Insert

Kif2c (Kif2c Cricetulus griseus (Chinese hamster))

Use

Tags

eGFP

Expression

Mammalian

Mutation

S92A , S106A , S108A, S112A , S186A, H530A, R534A…

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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