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pLentiGuide_GFP_sgEPAS1#1

Plasmid

#183119

Purpose

pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt10A-V5

Plasmid

#35939

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10A (WNT10A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAGM37443

Plasmid

#153218

Purpose

Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBSKΔB

Plasmid

#177268

Purpose

Backbone vector for STREAMING-tag knockin vector

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pCAFNF-tdTomato

Plasmid

#125575

Purpose

Flp-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS3

Plasmid

#145355

Purpose

Cell-free expression vector for Zika virus NS3 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS3

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET22b-T5-chi28TAG

Plasmid

#188984

Purpose

Express chimadanin with TAG at 28 position

Depositor

Han Xiao

Article

Chen et al Nat Commun. 2022 Sep 16;13(1):5434. doi:

Insert

Chimadanin with TAG at 28 position

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hRBM34-HA

Plasmid

#165464

Purpose

Human RBM34 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

RNA binding motif protein 34 (RBM34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5B

Plasmid

#35912

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pGATOR

Plasmid

#64102

Purpose

Tet inducible, Gateway adapted ROSA26 targeting Vector

Depositor

Bill Skarnes

Article

Melidoni et al Proc Natl Acad Sci U S A. 2013 Oct 29;11

Insert

rTTA (Gt(ROSA)26Sor Synthetic)

Use

Mouse Targeting; Tet inducible

Tags

Expression

Mammalian

Mutation

Promoter

ROSA26 endogenous

Available since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-5 pHR: U6-Sasgv2CXCR4-3 CMV-EGFP

Plasmid

#84256

Purpose

Expresses optimized Sa sgCXCR4-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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SyGCaMP5G

Plasmid

#162519

Purpose

Synaptically targeted GCaMP5G

Depositor

Josef Kittler

Article

Al Awabdh et al Glia. 2016 Jul;64(7):1252-64. doi: 10.10

Insert

GCaMP5G

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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hBACCS2

Plasmid

#67625

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP

Plasmid

#84255

Purpose

Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKVC

Plasmid

#145349

Purpose

Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ACS2 Truncated

Plasmid

#13748

Depositor

John Denu

Article

Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

Insert

Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 39–682

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-MTS-FLAG-LOV*

Plasmid

#202207

Purpose

Expresses the photoproximity labeling catalyst LOV* fused to the mitochondria targeting sequence (MTS) in mammalian cells

Depositor

Thomas Muir

Article

Hananya et al Proc Natl Acad Sci U S A. 2023 Apr 18;12

Insert

Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, aa 1-24

Use

Tags

FLAG and LOV*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pT2ADW_2paRAF

Plasmid

#129654

Purpose

For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GFP2-NbV5

Plasmid

#201478

Purpose

BRET2 detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

GFP2-NbV5

Use

Tags

GFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Rluc8-NbV5

Plasmid

#201479

Purpose

BRET detection of V5-tagged protein

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

NbV5-Rluc8

Use

Tags

Rluc8

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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