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Plasmid#48228PurposesgRNA expression on PiggyBac vector with Neo-selectable markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only
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Level 1 P2 OsUbiP:Cas9:NosT
Plasmid#165424PurposeGoldenGate (MoClo) Level 1 Position 2 OsUbiP: Cas9: NosT cassetteInsertOs Ubiquitin promoter :: Cas9 :: NosTerminator
UseSynthetic BiologyTagsExpressionMutationPromoterOs Ubiquitin promoter :: Cas9 :: NosTerminatorAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-FAID
Plasmid#127885PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter FAID taggingDepositorInsertGFP-Puro-H3F-AID (PPP2CA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcF-1
Plasmid#131023PurposeSingle spacer array with spacer #1 for FnCas12a.DepositorInsertsingle spacer array for FnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterPJ23119Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15a-AsCas12f-apmR
Plasmid#171610PurposeAsCas12f1-based K. pneumoniae genome editingDepositorInsertAsCas12f1
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVCERC-5 Pvan-dcas9 (Sth3)
Plasmid#133319Purposeexpresses dcas9 from Streptococcus thermophilus #3; induced with vanillate; for homologous recombination at the vanillate locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus thermophilus #3)
UseUnspecifiedTagsExpressionMutationPromotervanillateAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b-2AeGFP
Plasmid#121945PurposeMammalian expression, Genome editingDepositorInsertAaCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTAJAK-71 (pESC-NatMXsyn-USER)
Plasmid#78232PurposeEmpty vector, for the insertion of gRNA expression cassettes into the vector.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pM423
Plasmid#172408PurposeExpresses sfGFP (1-10) and MCP-GFP11DepositorInsertMCP, bipartite sfGFP
UseCRISPR and LentiviralTagssfGFP {1-10}ExpressionMammalianMutationPromoterhPGKAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
UseTagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-H3C2
Plasmid#207780PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.DepositorArticleInsertsgRNA Targeting C-terminus of H3C2 (H3C2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-sg-tdTomato90/816
Plasmid#179918PurposeDouble cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 90 and 816.DepositorInserttdTomato sgRNA
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterU6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AkCas12b-2AeGFP
Plasmid#121946PurposeMammalian expression, Genome editingDepositorInsertAkCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1821_pCR8-SMN2-DN-RG6-SA
Plasmid#118655PurposeTransient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway DonorDepositorInsertSMN2-DN-RG6-SA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-dSa-VPR mini.-syn pA
Plasmid#99689PurposeExpresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signalDepositorArticleInsertdCas9
UseAAVTagsVPR miniExpressionMutationdead Cas9PromoterAvailable sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTC223
Plasmid#70019PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7DepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
EC2_10_dCas9_VPR_HC_sgRNA
Plasmid#163712PurposeYeast high copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
UseTagsExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB6627
Plasmid#106159PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistanceDepositorInsertunknown
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pU1/Sm/3' Box_(GLuc)_TOP1
Plasmid#68437PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.DepositorInsertTOP1 construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U1Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only