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Plasmid#146025PurposeMammalian Expression vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only
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csr-1_son-1_sGFP
Plasmid#191561PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and sGFPExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-K650Q
Plasmid#191756PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1948A>C (p.K650Q) + silent mutation c.1938C&…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_mGFP-FGFR3-Y373C
Plasmid#191754PurposeExpression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-Y373C mutation (associated with TD1) (target mutation: c.1118A>G ; silent mutation added on purpose: c.1110C>A)DepositorInsertmGFP-FGFR3IIIc
UseTagsmGFPExpressionMammalianMutationc.1118A>G, p.Y373C + c.1110C>A (silent muta…PromoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-tdTomato-sg
Plasmid#194722PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomatoDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET-A457P
Plasmid#193365Purposeexpression of mutated human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (A457P mutation) (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationA457P (GCC to CCC)PromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-INS-sg
Plasmid#194719PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINSDepositorArticleInsertAsCpf1 (INS Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-MAFB-sg
Plasmid#194718PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFBDepositorArticleInsertAsCpf1 (MAFB Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-DD-pEGFP-C1
Plasmid#177429PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationThreonine 33 and Serine 35 replaced with aspartic…PromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-AA-pEGFP-C1
Plasmid#177430PurposeTo express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with 2 sites of phosphorylation mutated to alanine. T33A and S35A mutations made to Addgene #166737.DepositorInsertBik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
UseTagsVenusExpressionMammalianMutationThreonine 33 and Serine 35 replaced with alaninePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-CBS (H65R)
Plasmid#182924PurposeMammalian expression plasmid for human CBS mutant H65RDepositorInsertCystathionine Beta-Synthase (CBS Human)
UseTagseGFPExpressionMammalianMutationchanged histidine 65 to argininePromoterCMVAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Bid-GFP
Plasmid#191938PurposeExpression of BID-GFP chimeraDepositorInsertBid-GFP (Bid Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-mScarlet-AMPKα2
Plasmid#192451PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit.DepositorInsertmScarlet-AMPKalpha2 (Prkaa2 Rat)
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV VIVIT-GFP
Plasmid#188707PurposeLentivirus transfer plasmid encoding VIVIT-GFPDepositorInsertVIVIT-GFP
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Fis1-DTM
Plasmid#191950PurposeExpression of a Fis1-GFP chimera lacking the C-terminal 27 residues (Fis1-DTM)DepositorInsertFis1-DTM
UseTagsExpressionBacterialMutationFis1 gene lacking the C-terminal 27 residuesPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-eGFP
Plasmid#192583PurposeAAV-vector for bidirectional optogenetic manipulation of excitatory neuronsDepositorInsertsomBiPOLES
UseAAVTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterCaMKIIa(0.4)Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505:GFP
Plasmid#193001PurposePlasmid to generate a transgenic Hydra Zic4 reporter lineDepositorInsertHydra Zic4 promoter
UseHydra transgenesis vectorTagsGFPExpressionMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1d63C + VP2C-GFPdeg
Plasmid#182481PurposeYIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-GFPdeg
deltaVP1d63C
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_msfGFP-SEPT7 Gmut2
Plasmid#180425Purposemammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFPDepositorInsertSEPTIN7 (SEPTIN7 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT7 H279DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i3 Gmut-msfGFP
Plasmid#180326Purposemammalian expression of human SEPT9_i3 Gmut fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v3 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i3 W502A, H512DPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only