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Addgene
Showing: 6621 - 6640 of 16025 results
  1. pLVX-EGFP-C3-Cep123

    Plasmid
    #73330
    Purpose
    Lentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP tag
    Depositor
    Insert
    Cep123 (CEP89 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  2. p663-UBC-MTS-miniTurbo-V5_IDG-K

    Plasmid
    #214456
    Purpose
    Gateway destination clone of MTS-miniTurbo (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    MTS-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
  3. p663-UBC-MTS-TurboID-V5_IDG-K

    Plasmid
    #214457
    Purpose
    Gateway destination clone of MTS-TurboID (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    MTS-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
  4. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-M566T

    Plasmid
    #218832
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant M566T for BioID experiments.
    Depositor
    Insert
    TLK1 M566T (TLK1 Human)
    Use
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    M566T
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA5/FRT/TO-N-FLAG-hBirA*-TLK1-Q479E

    Plasmid
    #218833
    Purpose
    Expresses N-terminal BirA*-tagged human TLK1 variant 5 mutant Q479E for BioID experiments.
    Depositor
    Insert
    TLK1 Q479E (TLK1 Human)
    Use
    Tags
    FLAG-BirA*
    Expression
    Mammalian
    Mutation
    Q479E
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. CMYA5tnC/pDEST26-N-FLAG

    Plasmid
    #98130
    Purpose
    Express CMYA5 (aa 3859 – 4069) with a N-terminal FLAG tag in pDEST26 mammalian expression vector
    Depositor
    Insert
    CMYA5 (CMYA5 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
  7. AMPKA2

    Plasmid
    #36194
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    AMPKA2 (PRKAA2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    contains KINASE DOMAIN only
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  8. SUV420H2

    Plasmid
    #32864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    SUV420H2 (KMT5C Human)
    Use
    Tags
    His tag (with TEV protease)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
  9. Cin85-3SH3-pCMV6-AN-GFP

    Plasmid
    #51137
    Purpose
    Express turbo GFP tagged three SH3 domains of Cin85 (truncation) in mammalian cells
    Insert
    Cin85 (SH3KBP1 Human)
    Use
    Tags
    TurboGFP
    Expression
    Mammalian
    Mutation
    contains 3 SH3 domains
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  10. pFRT-TODestRFP_HuC

    Plasmid
    #65765
    Purpose
    expresses RFP-tagged HuC in mammalian cells
    Depositor
    Insert
    ELAVL3 (ELAVL3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Availability
    Academic Institutions and Nonprofits only
  11. pcDNA3.1/hpa5

    Plasmid
    #59972
    Purpose
    integrin a5 wild-type headpiece expression (a5 beta-propeller and a5 thigh domain)
    Depositor
    Insert
    human integrin a5 headpiece (ITGA5 Human)
    Use
    Tags
    acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Availability
    Academic Institutions and Nonprofits only
  12. p667-UBC-PKMYT1-V5_IDG-K

    Plasmid
    #135248
    Purpose
    Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    PKMYT1-V5 (PKMYT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
  13. p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K

    Plasmid
    #158161
    Purpose
    Gateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    2xV5-miniTurbo-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
  14. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Availability
    Academic Institutions and Nonprofits only
  15. pCAG2-EGFP-C-IFT122

    Plasmid
    #218737
    Purpose
    Expresses C-terminally EGFP-tagged IFT122 in mammalian cells
    Depositor
    Insert
    IFT122 (IFT122 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
  16. N-SF-TAP-DEST-TLK2-D592V

    Plasmid
    #218835
    Purpose
    Expresses N-terminal Strep-FLAG-tagged human TLK2 mutant D592V (kinase dead).
    Depositor
    Insert
    TLK2 Q592V (kinase dead) (TLK2 Human)
    Use
    Tags
    FLAG-STREP
    Expression
    Mammalian
    Mutation
    Q592V
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  17. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  18. pAcGFP-N1_CBPdAL

    Plasmid
    #221910
    Purpose
    Transient expression of GFP-tagged CBPdeltaAL in mammalian cells
    Depositor
    Insert
    CBPdeltaAL (CREBBP Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    deletion of the AL (aa 1558-1607); insertion at t…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 6621 - 6640 of 16025 results