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pBbA5k-acrAB-sfGFP

Plasmid

#195516

Purpose

Inducible expression vector for transcriptionally fused acrAB and sfGFP.

Depositor

Mary Dunlop

Article

Wen et al Sci Rep. 2018 Oct 11;8(1):15120. doi: 10

Insert

acrAB

Use

Synthetic Biology

Tags

sfgfp

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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VPS13D(∆ATG2-C/PH)^EGFP

Plasmid

#194004

Purpose

Deletion of DHPH domain of VPS13D

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D (VPS13D Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

early stop codon before DHPH

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-itis pBE-nt

Plasmid

#195343

Purpose

Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed nonsense non-targeting gRNA

Depositor

Alexis Komor

Article

Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

Inserts

ecTadA(8e)-SpCas9-NG
gRNA gtgcacgacgccgtatgcga

Use

CRISPR

Tags

Expression

Mutation

SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…

Promoter

Lac and ProC

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-19 TC-sfGFP-tag3

Plasmid

#194633

Purpose

fluorescent protein with cationic peptide tag and tetracysteine tag

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-sfGFP-K(GGSKKRKKR)3

Use

Tags

KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGCCPGCCGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-9 GFP(+6)-tag3

Plasmid

#194624

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(+6)-(GGSKKRKKR)3

Use

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-8 GFP(+6)-tag2

Plasmid

#194623

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(+6)-(GGSKKRKKR)2

Use

Tags

GGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-7 GFP(+6)-tag1

Plasmid

#194622

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(+6)-GGSKKRKKR

Use

Tags

GGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-6 GFP(0)-tag3

Plasmid

#194621

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(0)-(GGSKKRKKR)3

Use

Tags

GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-5 GFP(0)-tag2

Plasmid

#194620

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(0)-(GGSKKRKKR)2

Use

Tags

GGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-4 GFP(0)-tag1

Plasmid

#194619

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

GFP(0)-GGSKKRKKR

Use

Tags

GGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-2 sfGFP-tag2

Plasmid

#194617

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-K(GGSKKRKKR)2

Use

Tags

KGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-1 sfGFP-tag1

Plasmid

#194616

Purpose

fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

sfGFP-KGGSKKRKKR

Use

Tags

KGGSKKRKKR and MGHHHHHGGA

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT GFP DAmP

Plasmid

#182511

Purpose

C-SWAT compatible vector for C-terminal tagging with GFP DAmP. GFP is immediately followed by the Hygromycin B (Hph) resistance cassette.

Depositor

Rob Mitra

Article

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

GFP

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBicaudalC_P

Plasmid

#147158

Purpose

Insect Expression of DmBicaudalC

Depositor

Elisa Izaurralde

Insert

DmBicaudalC (BicC Fly)

Use

Tags

Expression

Insect

Mutation

12 silent and three non silent mutattions I331T, …

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-EGFP_P

Plasmid

#147174

Purpose

Insect Expression of EGFP

Depositor

Elisa Izaurralde

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-FLuc-3BoxB_O

Plasmid

#147078

Purpose

Mammalian Expression of 3BoxB

Depositor

Elisa Izaurralde

Insert

3BoxB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-FLuc-7BoxB_O

Plasmid

#147080

Purpose

Mammalian Expression of 7BoxB

Depositor

Elisa Izaurralde

Insert

7BoxB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGrowl_O

Plasmid

#147066

Purpose

Insect Expression of DmGrowl

Depositor

Elisa Izaurralde

Insert

DmGrowl (lost Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-CTAP_J

Plasmid

#146648

Purpose

Insect Expression of EGFP

Depositor

Elisa Izaurralde

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-NTAP-EGFP_J

Plasmid

#146653

Purpose

Insect Expression of EGFP

Depositor

Elisa Izaurralde

Insert

EGFP

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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