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pNTKV- Esrrb-KI-3'UTR-2A-TagBFP-RBGpA

Plasmid

#128835

Purpose

Knock-in of TagBFP into mouse Esrrb locus

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

tagBFP into Esrrb locus (Esrrb Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Flag-Avi-ires-CHD5

Plasmid

#68878

Purpose

Transient mammalian expression of CHD5 for BirA tagging

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG/AVI

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only
pX330-Esrrb gRNA

Plasmid

#128841

Purpose

gRNA for targeting mouse Esrrb locus using CRISPR-cas technique

Depositor

Yossi Buganim

Article

Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

Insert

Esrrb gRNA (Esrrb Mouse)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-GST-CHD5(Chromos)-FRAG

Plasmid

#68875

Purpose

IPTG inducible bacterial expression of CHD5 fragment

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

only the two chromodomains of CHD5

Promoter

tac

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized

Plasmid

#172346

Purpose

Expression of FLAG-tagged, codon-optimized, truncated SRSF3

Depositor

Sujatha Jagannathan

Article

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized, truncated at amino acid 114 with…

Promoter

EF-1a

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221 - myc ULK1 k46I

Plasmid

#27624

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 K46I (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Expression

Mutation

Initial M from ULK1 removed. Silent mutation at A…

Promoter

Available since

Feb. 18, 2011

Availability

Academic Institutions and Nonprofits only
pDONR221 - myc ULK1 4SA

Plasmid

#27625

Depositor

Reuben Shaw

Article

Egan et al Science. 2010 Dec 23. ():.

Insert

mouse myc ULK1 4SA (Myc Mouse)

Use

Gateway donr vector

Tags

myc

Expression

Mutation

S467A, S555A, T574A, S637A. Initial M from ULK1 r…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-FLAG-CHD5-WT-331-672aa

Plasmid

#68870

Purpose

Mammalian expression of CHD5 histone binding domain (331-672aa)

Depositor

Adrian Bracken

Article

Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

Insert

CHD5 (CHD5 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

331-672aa

Promoter

CMV

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only
pDV18 [punc-47::SNG-1::CRY2olig(535)]

Plasmid

#197598

Purpose

Expression of SNG-1::CRY2olig(535) in GABAergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

Insert

Synaptogyrin-CRY2 (E490G + residues 1-535) (CRY2, sng-1 Nematode, Mustard Weed)

Use

Tags

Expression

Worm

Mutation

E490G

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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