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pTALEN-STAR-G

Plasmid

#87525

Purpose

Destination vector for TALEN construction

Depositor

Steve Pollard

Article

Gogolok et al Sci Rep. 2016 Sep 12;6:33209. doi: 10.10

Type

Empty backbone

Use

Synthetic Biology and TALEN

Tags

Expression

Mutation

Promoter

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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mIFP12-Farnesyl-5

Plasmid

#56251

Purpose

Localization: Plasma Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Farnesyl (HRAS Human)

Use

Tags

mIFP12

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCardinal-Farnesyl-5

Plasmid

#56159

Purpose

Localization: Plasma Membrane, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Farnesyl (HRAS Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192239

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192242

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3f-knockin-homology arm-mAID-mCherry2-Hygro (EIF3F Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago2

Plasmid

#21538

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

The first 7aa of this Ago2 protein are different …

Promoter

Available since

Sept. 21, 2009

Availability

Academic Institutions and Nonprofits only

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Cas12aUltra_5XNLS

Plasmid

#218775

Purpose

His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

Depositor

Ross Wilson

Article

Sahu et al bioRxiv 2024.07.14.603391

Insert

5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a

Use

CRISPR

Tags

6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

Expression

Bacterial

Mutation

Nucleotides mutations were made to encode for arg…

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-synaptoPAC-minWPRE

Plasmid

#153085

Purpose

Expresses synaptoPAC, a fusion of synaptophysin, mScarlet, and bPAC in neurons

Depositor

Dietmar Schmitz

Article

Oldani et al J Neurochem. 2021 Feb;156(3):324-336. do

Inserts

bPAC(WT)
Synaptophysin (Syp Rat)

Use

AAV

Tags

His tag, mScarlet, and myc tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Ago1

Plasmid

#21533

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-PIWI(aa478-860)

Plasmid

#21543

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa478-860

Promoter

Available since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-PAZ(aa1-480)

Plasmid

#21529

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

contain only aa1-480

Promoter

Available since

July 29, 2009

Availability

Academic Institutions and Nonprofits only
pZ_P-GAP-ACS1

Plasmid

#126712

Purpose

yeast plasmid complementing ACS1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

acs1

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]-Gwpep(Mm)

Plasmid

#188589

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler

Use

Tags

FLAG-Myc and GWpep mm

Expression

Insect

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[C399-625]-Gwpep(Dm)

Plasmid

#188590

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler

Use

Tags

FLAG-Myc and Gwpep dm

Expression

Insect

Mutation

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ALD1

Plasmid

#126711

Purpose

yeast plasmid complementing ALD1 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

ald1

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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pZ_P-GAP-ADH3

Plasmid

#126710

Purpose

yeast plasmid complementing ADH3 from Komagataella phaffii

Depositor

Menghao Cai

Article

Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

Insert

adh3

Use

Tags

6xHis,Myc

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 19, 2019

Availability

Academic Institutions and Nonprofits only

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FLINC-AKAR1(TA)-CAAX

Plasmid

#87706

Purpose

Negative-control mutant of FLINC-AKAR1-CAAX.

Depositor

Jin Zhang

Article

Mo et al Nat Methods. 2017 Apr;14(4):427-434. doi

Insert

FLINC-AKAR1(TA)-CAAX

Use

Tags

6xHis, C-terminal targeting sequence from Kras, T…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_XIAP_WT

Plasmid

#82170

Purpose

Gateway Donor vector containing XIAP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XIAP (XIAP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PYGL_WT_V5

Plasmid

#82991

Purpose

Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PYGL (PYGL Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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