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pET28b-BE1

Plasmid

#73018

Purpose

Expresses BE1 with N-terminal His6 tag in E. Coli

Depositor

David Liu

Article

Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

Insert

BE1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 RNA Polymerase

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pET12a-PAPI

Plasmid

#19961

Depositor

Jonathan Tait

Article

Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Insert

Annexin V (ANXA5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pET32a-TRXtag

Plasmid

#11516

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

None - thioredoxin is vector encoded (trxA )

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

vector modified; removed N-terminal His tag by di…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pET41a-MED19

Plasmid

#15430

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Use

Tags

GST and His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a iGluh

Plasmid

#119830

Purpose

Production of recombinant iGluSnFR E25A high affinity variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluh

Use

Tags

GST

Expression

Bacterial

Mutation

E25A

Promoter

T7

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Use

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Rat, Human, Bacillus phage PBS2)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO3

Plasmid

#53143

Purpose

E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO3 (SUMO2 Human)

Use

Tags

Expression

Bacterial

Mutation

mature form (amino acids 1-93)

Promoter

T7

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pETM-41-HsNot6_N

Plasmid

#147011

Purpose

Bacterial Expression of HsNot6

Depositor

Elisa Izaurralde

Insert

HsNot6 (CNOT6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Use

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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YPet-pBAD

Plasmid

#54860

Purpose

Localization: Protein Expression Vector , Excitation: 513, Emission: 527

Depositor

Patrick Daugherty, Michael Davidson

Article

Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

Type

Empty backbone

Use

Tags

6xHis and Ypet

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-DONSON

Plasmid

#208797

Purpose

Xenopus laevis His6-Myc-DONSON wild type

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pETx-SR6v15

Plasmid

#176212

Purpose

Bacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6v15

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36β

Plasmid

#214344

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36B (IL36B Human)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Pro-IL-36α

Plasmid

#214343

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL36A (IL36A Human)

Use

Tags

His-TEV and Myc

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET21-Flavidin

Plasmid

#89881

Purpose

Fluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labeling

Depositor

Mark Howarth

Article

Jacobsen et al Cell Chem Biol. 2017 Jul 17. pii: S2451-

Insert

Streptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag

Use

Tags

6 glutamate tag (C terminal on insert)

Expression

Bacterial

Mutation

K80R, K121R, K132R, K134R

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-mNG-NL

Plasmid

#169106

Purpose

Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - mNeonGreen - NanoLuc

Use

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pETx-SR6c3

Plasmid

#176211

Purpose

Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

SR6c3

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge