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Plasmid#89367PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmyo-3Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2048-csnDEG
Plasmid#89368PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression controlled by Posm-6 (ciliated sensory neuron specific)DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPosm-6Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOD2044-intDEG
Plasmid#89366PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pelt-2 (intestine specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPelt-2Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3xVHH05-DsRed
Plasmid#171580PurposeFor scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-C-3x127D01-DsRed
Plasmid#171578PurposeFor scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-vsfGFP-0
Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3xVHH05-DsRed
Plasmid#171581PurposeFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3xVHH05 (UBE2J1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-actin
Plasmid#108968Purposeexpresses BC2-tag-actin in mammalian cellsDepositorInsertbeta-actin (ACTB Human)
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 AC30_eGFP
Plasmid#82715PurposeExpresses a GFP-tagged recombinant adenylyl cyclase.DepositorUseTagsFLAG purification tag and eGFPExpressionBacterial and InsectMutationPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-lamin
Plasmid#108972Purposeexpresses BC2-tag-lamin in mammalian cellsDepositorInsertlamin B1 (LMNB1 Human)
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)
Plasmid#160742PurposeExpresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.DepositorInsertHA-nEPEA-(EAAAK)4-OGT(4,K852A)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192788PurposeaGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody enhancer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCre-MBP4-WPRE
Plasmid#193919PurposeExpresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)DepositorInsertCCre-MBP4
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCre-MBP5-WPRE
Plasmid#193918PurposeExpresses one of the components of Cre-DOR_N5C4 (RFP-dependent Cre)DepositorInsertNCre-MBP5
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOD2087-trnDEG
Plasmid#89369PurposeMosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).DepositorInsertvhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
UseTargeting vector for mos1 transposon mediated sin…TagsvhhGFP4 (GFP nanobody)ExpressionMutationPromoterPmec-18Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-CCre-MBP1-WPRE
Plasmid#193917PurposeExpresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)DepositorInsertCCre-MBP1
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-NCre-MBP6-WPRE
Plasmid#193916PurposeExpresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)DepositorInsertNCre-MBP6
UseAAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBC2T-LC3B
Plasmid#112026Purposeexpresses BC2-tag-LC3B in mammalian cellsDepositorInsertLC3B (MAP1LC3B Human)
UseTagsBC2-tagExpressionMammalianMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-HAPPID1
Plasmid#204626PurposeExpresses the human anti-Phl p 7 IgD/λ antibody 102.1F10 (HAPPID1) in mammalian cells. HAPPID1 binds to the grass pollen allergen Phl p 7 with subnanomolar affinity.DepositorInsertsHAPPID1 heavy chain
HAPPID1 light chain
UseTagsExpressionMammalianMutationPromotermouse elongation factor 1 alpha and rat elongatio…Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only