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pXL_dSSN3_EF1a-tNGFR-2A-Puro

Plasmid

#201113

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 3

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN4_EF1a-tNGFR-2A-Puro

Plasmid

#201114

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 4

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN5_EF1a-tNGFR-2A-Puro

Plasmid

#201115

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 5

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN6_EF1a-tNGFR-2A-Puro

Plasmid

#201116

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 6

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pXL_dSSN7_EF1a-tNGFR-2A-Puro

Plasmid

#201117

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 7

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXL_dSSN8_EF1a-tNGFR-2A-Puro

Plasmid

#201118

Purpose

Dual barcoding cassettes for SSN-seq; Barcode Set 8

Depositor

Pawel Mazur

Article

Lu et al Nat Biomed Eng. 2023 Aug 31. doi: 10.103

Inserts

SSN.guide
SSN.F30
tNGFR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a, Human U6, and Mouse U6

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pgTS40

Plasmid

#169630

Purpose

pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

U6-driven gRNA expression and PCAG-driven SpCas9 expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 / PCAG

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1041

Plasmid

#169619

Purpose

Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc and mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pVH228-Tier1-OVanO2-PCMVmin2-SEAP

Plasmid

#169564

Purpose

Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

vanillic acid-controlled SEAP production

Use

Tags

Expression

Mammalian

Mutation

Promoter

VanO2-PCMVmin

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1040

Plasmid

#169618

Purpose

Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven expression of secreted Nluc and mTagBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PPGK

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS1039

Plasmid

#169617

Purpose

Tier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven expression of SEAP and iRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2365-Tier1-PhCMV-TetR

Plasmid

#169566

Purpose

Tier-1 vector encoding PhCMV-driven tetracycline dependent regulator protein TetR (PhCMV-TetR-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven tetracycline repressor protein class B from transposon Tn10

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

Plasmid

#169588

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-2

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH63-Tier1-PMLP-Citrine_p2a_SEAP

Plasmid

#169557

Purpose

Tier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PMLP-driven citrine-P2A-SEAP fusion construct

Use

Tags

Expression

Mammalian

Mutation

Promoter

PMLP

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2366-Tier1-PhCMV_F-type

Plasmid

#169567

Purpose

Tier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven F-type VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH255-Tier1-OTetO7-PCMVmin1-SEAP

Plasmid

#169587

Purpose

Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

tetracycline-controlled SEAP expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO7-PCMVmin-1

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pVH65-Tier1-PTK-Citrine_p2a_SEAP

Plasmid

#169559

Purpose

Tier-1 vector encoding PTK-driven Citrine-p2A-SEAP expression (PTK-Citrine-p2A-SEAP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PTK-driven citrine-P2A-SEAP fusion construct

Use

Tags

Expression

Mammalian

Mutation

Promoter

PTK

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2388-Tier1-PhCMV-p2a_Ypet

Plasmid

#169525

Purpose

Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused yellow fluorescent protein Ypet

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2387-Tier1-PhCMV-p2a_mTagBFP2

Plasmid

#169524

Purpose

Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2389-Tier1-PhCMV-p2a_mRuby

Plasmid

#169526

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused enhanced yellow fluorescent protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

PhCMV

Available since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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