Addgene

We narrowed to 2,043 results for: Xpa

Showing: 641 - 660 of 2043 results

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Tags

Expression

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMK459 (dTAG-BromoTag-mAID-EGFP)

Plasmid

#214387

Purpose

dTAG-BromoTag-mAID-EGFP reporter (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

dTAG-BromoTag-mAID-EGFP reporter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMS178

Plasmid

#215678

Purpose

Empty repair plasmid for ChrII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxP + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMS18

Plasmid

#215675

Purpose

Cas9 + guide plasmid for inserting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GAAATCGCCGACTTGCGAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS62

Plasmid

#215676

Purpose

Cas9 + guide plasmid for inserting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCGTTCTTCCGTTCTCGGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMS114

Plasmid

#215677

Purpose

Empty repair plasmid for ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + loxN + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS59

Plasmid

#215680

Purpose

Cas9 + guide plasmid targeting ChrI split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS158

Plasmid

#215679

Purpose

Empty repair plasmid for ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

5'HA + MCS + lox2272 + rps-0p::5'ΔHygR

Use

CRISPR and Cre/Lox

Tags

Expression

Worm

Mutation

5' ∆HYGR encodes aa1-226

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMS77

Plasmid

#215681

Purpose

Cas9 + guide plasmid targeting ChrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

eef-1A.1p::Cas9 + U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.1

Plasmid

#208306

Purpose

Expresses ABE-RA4.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.2

Plasmid

#208307

Purpose

Expresses ABE-RA4.2 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.2-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.3

Plasmid

#208308

Purpose

Expresses ABE-RA4.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.3-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.4

Plasmid

#208309

Purpose

Expresses ABE-RA4.4in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.4-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA4.5

Plasmid

#208310

Purpose

Expresses ABE-RA4.5 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.5-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE-RA4.6

Plasmid

#208311

Purpose

Expresses ABE-RA4.6 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA4.6-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

P48A, R51H, I76F, A106V, D108G, K110R, T111H, D11…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.0

Plasmid

#208312

Purpose

Expresses ABE-RA5.0 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.0-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.1

Plasmid

#208313

Purpose

Expresses ABE-RA5.1 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.1-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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ABE-RA5.3

Plasmid

#208314

Purpose

Expresses ABE-RA5.3 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.3-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, H36L, R47K, P48A, R51L, I76F, V82S, A106V, …

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
ABE-RA5.4

Plasmid

#208315

Purpose

Expresses ABE-RA5.4 in mammalian cells

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA-RA5.4-SpCas9 D10A

Use

Tags

Expression

Mammalian

Mutation

W23R, R47K, P48A, R51L, I76Y, V82S, A106V, D108G,…

Promoter

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge