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pEF1a-T2-NLuc

Plasmid

#135938

Purpose

Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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OA959C

Plasmid

#104968

Purpose

Expresses anti-ZIKV transgene in Ae. aegypti

Depositor

Omar Akbari

Article

Buchman et al Proc Natl Acad Sci U S A. 2019 Feb 5. pi

Insert

Anti-Zika Transgene

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pTSAR3.3t

Plasmid

#210255

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

DsRed
superfolder GFP

Use

Tags

ssra

Expression

Bacterial

Mutation

Promoter

ipaH7.8p and rpsM

Available since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-GpNLuc

Plasmid

#135949

Purpose

Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

GpNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-OgNLuc

Plasmid

#135951

Purpose

Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

OgNLuc LumiFluor

Use

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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LT3REVIR

Plasmid

#111176

Purpose

Tet-ON miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pDS-RED-C1-Beclin1

Plasmid

#24405

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Use

Tags

DsRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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pEB2-mScarlet

Plasmid

#104006

Purpose

Plasmid encoding mScarlet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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OA984-HA

Plasmid

#120362

Purpose

Expresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporter

Depositor

Omar Akbari

Article

Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

Insert

1C19 his-tagged single chain antibody

Use

Unspecified

Tags

Expression

Mutation

Promoter

AAEL010782 carboxypeptidase A (CPA) gene Promoter

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Use

Tags

Expression

Insect

Mutation

Promoter

vasa

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry2-L

Plasmid

#104003

Purpose

Plasmid encoding mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry2-L

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNP1 tdTomato toehold switch

Plasmid

#107354

Purpose

Expresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

toehold switch 5 + tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-chimpTRIM5-FOS

Plasmid

#79065

Purpose

Express chimpanzee TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(chimpanzee)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-whiteCopyCatcher

Plasmid

#174062

Purpose

CopyCatcher insertion donor for the white locus

Depositor

Ethan Bier

Article

Li et al Nat Commun. 2021 May 11;12(1):2625. doi:

Insert

white (w Fly, Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJM553 ZF10x6-C 3xFLAG-NLS-DsDed-ZF1-PEST in TUPV2

Plasmid

#161540

Purpose

Inducible expression of 3xFLAG-NLS-DsDed-ZF1-PEST under the ZF10x6-C promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsRed-ZF1-PEST

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

ZF10x6-C

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEB2-mScarlet-I

Plasmid

#104007

Purpose

Plasmid encoding mScarlet-I

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mScarlet-I

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGE AVIKEF… N6A, R…

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAVTRE3_tdTomato

Plasmid

#135178

Purpose

AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

Depositor

Tetsuo Yamamori

Article

Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

Inserts

tdTomato
TRE3G

Use

AAV

Tags

Expression

Mammalian

Mutation

a variant of DsRed

Promoter

TRE3G

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCFD5-U6-3-t-attP40

Plasmid

#133561

Purpose

gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

attP40 region guide RNAs

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

U6

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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