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p3259 pCMV hUBC9 mt HA

Plasmid

#14439

Depositor

Peter Howley

Article

Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

Insert

UBC9 C93S (UBE2I Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Cys93Ser mutation (TGC changed to AGC)

Promoter

CMV

Available since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-LAC

Plasmid

#64965

Purpose

mini-Tn7 vector with Ptac expression cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDonor_Tn[himar1C9]

Plasmid

#213906

Purpose

Mariner transposon with ATc-inducible himar1C9 transposase

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and ATc-inducible himar1C9 transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET_RTX

Plasmid

#102787

Purpose

Expresses the Reverse Transcriptase gene

Depositor

Andrew Ellington

Article

Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

Insert

reverse transcription xenopolymerase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter + lac operator

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pYes2-TaPCS1

Plasmid

#49767

Purpose

Expresses wheat phytochelatin synthase (TaPCS1) in Saccharomyces Cerevisiae

Depositor

Julian Schroeder

Article

Clemens et al EMBO J. 1999 Jun 15;18(12):3325-33.

Insert

TaPCS1 (PCS1 Triticum aestivum)

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only
pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

Plasmid

#40019

Depositor

Vincent Noireaux

Article

Shin et al J Biol Eng. 2010 Jun 24;4:8.

Insert

deGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available since

Oct. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa3-IRES-GFP

Plasmid

#33009

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A3 (Foxa3 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pCAG-Noggin

Plasmid

#163586

Purpose

Expresses Noggin in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Nog (Nog Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGFP-HBx

Plasmid

#24931

Depositor

Xin Wang

Article

Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

Insert

HBx

Use

Tags

EGFP and HA

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Use

Tags

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGFPHYG2

Plasmid

#30173

Depositor

Lalita Ramakrishnan

Article

Cosma et al Infect Immun. 2006 Jun . 74(6):3125-33.

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

GFP

Expression

Mutation

GFPmut3 allele has improved brightness.

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Use

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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EasyFusion mCherry

Plasmid

#112849

Purpose

General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RTP1S

Plasmid

#149364

Purpose

Expresses RTP1S in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

RTP1S (Rtp1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T

Plasmid

#64957

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET28a-ChmN

Plasmid

#221348

Purpose

Express ChmN in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmN

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pFLAG-XBP1u-FLuc

Plasmid

#31239

Depositor

Jimtong Horng

Article

Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

Insert

FLAG-XBP1u-FLuc (XBP1 Human)

Use

Tags

Flag and Fluc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2011

Availability

Academic Institutions and Nonprofits only
C1-CFP-Lyn11

Plasmid

#155234

Purpose

membane marker

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lyn11 membrane targeted CFP. Membrane control

Use

Tags

CFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
EcYbbN pET15b

Plasmid

#60690

Purpose

Recombinant protein expression of YbbN

Depositor

Mark Wilson

Article

Lin et al J Biol Chem. 2011 Jun 3;286(22):19459-69

Insert

YbbN

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only
pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TRX1 flag C35S

Plasmid

#21285

Depositor

Wang Min

Article

Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

Insert

TRX1 (TXN Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

C35S

Promoter

Available since

July 30, 2009

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2S

Plasmid

#15807

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2S (UBE2S Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-RFP

Plasmid

#22910

Depositor

Edward Callaway

Article

Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

Insert

Chicken Beta-actin promoter driving DsRedExpress

Use

AAV; Adeno-associated virus

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
mRFP1-pBAD

Plasmid

#54667

Purpose

Localization: Protein Expression Vector , Excitation: 584, Emission: 607

Depositor

Robert Campbell, Michael Davidson, Roger Tsien

Article

Campbell et al Proc Natl Acad Sci U S A. 2002 Jun 11;99

Type

Empty backbone

Use

Tags

6xHis and mRFP1

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pSWAP_Entry_H2_beta_CM

Plasmid

#184870

Purpose

pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGL2-eNOSPromoter-Luciferase

Plasmid

#19297

Depositor

Charles Lowenstein

Article

Arnet et al J Biol Chem. 1996 Jun 21. 271(25):15069-

Insert

endothelial nitric oxide synthase promoter (NOS3 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pUC18-mini-Tn7T-Gm-lacZ

Plasmid

#63120

Purpose

mini-Tn7 lacZ transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pET15-UbE2W

Plasmid

#15809

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2W (UBE2W Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pPS2977

Plasmid

#8927

Depositor

Pamela Silver

Article

Devroe et al J Biol Chem. 2004 Jun 4. 279(23):24444-5

Insert

NDR1 (STK38 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

see comments

Promoter

Available since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pDEST17-UbE2H

Plasmid

#15752

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2H (UBE2H Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pFCM1

Plasmid

#64948

Purpose

source of CmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-cat-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBSKΔB-CAG-MCP-tdiRFP670-IRES-Neo

Plasmid

#62345

Purpose

Expresses MCP-tdiRFP670 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Nucleic Acids Res. 2015 Jun 19. pii: gkv

Insert

MCP-tdiRFP670

Use

Tags

HA and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pNM3725

Plasmid

#154113

Purpose

UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

UAS 11X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsTNRC6A

Plasmid

#25035

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

TNRC6A (TNRC6A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(-)-HA-SrtAstrep (pKS77)

Plasmid

#50030

Purpose

Expression of cytosolic S. pyogenes SrtA in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

SrtA(strep)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-miR30

Plasmid

#20670

Depositor

Bryan Cullen

Article

Zeng et al Mol Cell. 2002 Jun . 9(6):1327-33.

Insert

miR-30 (MIR30A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
pNM3813

Plasmid

#154121

Purpose

mec-4 promoter driving lexA-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::lexA-L-QFAD::act-4 '3

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNM3811

Plasmid

#154120

Purpose

mec-4 promoter driving QF2 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::QF2::act-4 '3

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNM3727

Plasmid

#154115

Purpose

lexO 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

lexO 5X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pNM3726

Plasmid

#154114

Purpose

QUAS 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

QUAS 5X::∆pes-10::GFP-C1::tbb-2 3'

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-myc-USP10

Plasmid

#184034

Purpose

Expresses myc-USP10 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

Insert

USP10 (USP10 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMVf

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pSWAP_Entry_H1_alpha

Plasmid

#184865

Purpose

pSwap plasmid part with H1_alpha homology arms

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSWAP_Entry_HAIB

Plasmid

#184869

Purpose

For scarless integration into the chromosome, HAIB plasmid can be usedin place of the HA, INS and HB modules.

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pSWAP_Entry_H2_beta_GM

Plasmid

#184871

Purpose

pSwap plasmid part with H2_beta homology arms and gentamycin cassette

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

lacZalpha,ccdB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMito-iRFP720

Plasmid

#45464

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Use

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 Hygro EYFP H148Q/I152L

Plasmid

#25874

Depositor

Peter Haggie

Article

Galietta et al FEBS Lett. 2001 Jun 22. 499(3):220-4.

Insert

EYFP H148Q/I152L

Use

Tags

Expression

Mammalian

Mutation

EYFP from pEYFP (Clontech) with H148Q and I152L m…

Promoter

Available since

Aug. 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pTFUbiCre

Plasmid

#139670

Purpose

Expression cassette for Cre recombinase in T-DNA vector

Depositor

James Birchler

Article

Gaeta et al Chromosoma. 2013 Jun;122(3):221-32. doi:

Insert

Cre recombinase

Use

Tags

Expression

Plant

Mutation

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pTOX12

Plasmid

#127457

Purpose

Encodes Tse2 toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

tse2 toxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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