-
Plasmid#134914PurposesgRNA targeting GFP to be used in nanoblade systemDepositorInsertGFP
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet-I
Plasmid#104007PurposePlasmid encoding mScarlet-IDepositorInsertmScarlet-I
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
iOn-CAG∞MCS
Plasmid#154013PurposeExpression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoterDepositorInsertMCS
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mScarlet
Plasmid#104006PurposePlasmid encoding mScarletDepositorInsertmScarlet
UseLow copyTagsExpressionBacterialMutationMutations relative to DsRed (MSKGE AVIKEF… N6A, R…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-human APPL1
Plasmid#22198DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 21, 2009AvailabilityAcademic Institutions and Nonprofits only -
BLADE
Plasmid#134912PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades systemDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
mRFP-human APPL1
Plasmid#22202DepositorInsertAdaptor protein containing PH domain, PTB domain and Leucine zipper motif (APPL1 Human)
UseTagsmRFPExpressionBacterial and MammalianMutationPromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX304-VHLdeltaB-IRES-GFP
Plasmid#187642PurposeMutant VHL Expression vector with GFPDepositorInsertVHL (VHL Human)
UseLentiviralTagsExpressionMammalianMutationdeletion of amino acids 91 to 121PromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRuby3 Addgene
Plasmid#104005PurposePlasmid encoding mRuby3 AddgeneDepositorInsertmRuby3 Addgene
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP611 (MSKGEE LIKENMRMKVV…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFL His-mEos3.2-I3
Plasmid#113516PurposeExpression of His-Eos-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*His-mEos3.2ExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 CaRhAC T2A tDimer
Plasmid#101722Purpose- humanized - variant of pAAV hSyn YFP-CaRhAC Addgene # 101721– less reliable in hippocampal neuronsDepositorInsertsCatRhAC
red fluorescent protein
UseAAVTagsExpressionMutationE497K,C569DPromoterhuman Synapsin1 promotor and ribosomal skip seque…Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_WT
Plasmid#40202DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationWTPromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEB2-mRuby3
Plasmid#104004PurposePlasmid encoding mRuby3DepositorInsertmRuby3
UseLow copyTagsExpressionBacterialMutationMutations relative to eqFP611 (MSKGEE LIKENMRMKVV…PromoterproCAvailable sinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFudioFRTPSD-95TealW
Plasmid#134299PurposeExpresses PSD95 fused to Teal fluorescent protein upon coexpression with the recombinase flippase (Flp)DepositorInsertPSD-95-Teal (Dlg4 Rat, Mouse)
UseLentiviral; Flp/frtTagsTealExpressionMammalianMutationPromoterUbCAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTargetF-FRT
Plasmid#113637PurposeDerivative of pTargetF with gRNA targeting FRT siteDepositorInsertsgRNA-FRT
UseCRISPRTagsExpressionMutationPromoterpJ23119Available sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#113398Purposeexpresses gRNA for Cas9 FRT targettingDepositorInsertexo, beta, gam, sgRNA-FRT
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCHOP10(dLZ).pCDNA1
Plasmid#21900DepositorInsertCHOP10 (Ddit3 Mouse)
UseTagsExpressionMammalianMutationThe coding region of the murine CHOP-10 cDNA was …PromoterAvailable sinceOct. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn deltaSH3
Plasmid#16034DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationDelta SH3: deletion of amino acids 76-141PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_S146A
Plasmid#40205DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationS146APromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Fyn SH2:R176E
Plasmid#16035DepositorInsertc-Fyn (FYN Human)
UseTagsExpressionMammalianMutationSH2 point mutant: R176EPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_S146D
Plasmid#40206DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationS146DPromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaLZ_P2A_Hygro_Barcode
Plasmid#120509PurposeBarcoded lentiviral vector to express MYC deltaLZ in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaLZ (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of leucine zipper motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_T145A
Plasmid#40203DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationT145APromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_T145D
Plasmid#40204DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationT145DPromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
LR (hsp70I: cPLA2-mK2)
Plasmid#162564PurposeMeasuring cPLA2 activation in live cells after heat shock.DepositorUseGateway donor vectorTagsmK2ExpressionMutationPromotercmlc2Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3
Plasmid#113513PurposeExpression of His-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertmTQ2=mturquoise2 blue fluorescent gene
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMutationPromoterVillinAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
-
SUPERBLADE5
Plasmid#134913PurposeEmpty backbone for cloning sgRNA sequence to be used in nanoblades system (Optimized for increased genome editing efficiency via Chen B et al., 2013)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterU6Available sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Human, Budding Yeast)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pEB1N-LZ-LOV2(wt)
Plasmid#107614PurposeN-terminal half of π-EB1 with wild-type LOV2, no fluorescent tagDepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain and GCN4 leuc…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterCMVAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His beta4
Plasmid#16039DepositorInsertbeta4 (ITGB4 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1
Plasmid#162566PurposeUsed for the dual color labeling of cPLA2 and LaminB1 expression.DepositorUseTagsEGFP, P2A, and mKate2ExpressionMammalianMutationPromoterCMV and SV40Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHES795
Plasmid#87943PurposeConstitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertZif268 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Human, Budding Yeast)
UseTagsExpressionYeastMutationPromoterADH1 (crippled)Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
H11-mScarletSIIN
Plasmid#172438PurposeHipp11 targeting vector, expresses mScarlet-SIINFEKLDepositorInsertmScarlet-SIIN
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-FLEX(frt)-GCaMP6f-WPRE
Plasmid#118273PurposepAAV vector for flippase dependent GCaMP6f expression under the control of EF1a promoterDepositorInsertGCaMP6f
UseAAVTags6xHis (N terminal on insert), T7 epitope, and Xpr…ExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab beta Derp1-117
Plasmid#113639PurposeInsect cell expression vector for I-Ab beta chain fused to Der p 1 117-127 antigenic peptideDepositorInsertI-Ab beta Derp1-117 (H2-Ab1 Mouse)
UseTags6x His tag, fused to Der p 1 (117-127) peptide vi…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
H11-mScarletALG8
Plasmid#172440PurposeHipp11 targeting vector, expresses mScarlet-mALG8DepositorInsertmScarlet-mALG8
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK5 beta4 1355T
Plasmid#16038DepositorInsertbeta4 (ITGB4 Human)
UseTagsExpressionMammalianMutation1355T: amino acids 1-1355PromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_T145A/S146A
Plasmid#42623DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationT145A/S146APromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-SET-EMD (N-SET-EMD)
Plasmid#217765PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
UseTagsEGFPExpressionMammalianMutationEMD domain (aa 70-226)PromoterCMVAvailable sinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSET-EMD-EGFP (C-SET-EMD)
Plasmid#217764PurposeFluorescent reporter for ceramide (putative, mammalian expression)DepositorInsertSET (SET Human)
UseTagsEGFPExpressionMammalianMutationEMD domain (aa 70-226)PromoterCMVAvailable sinceJune 12, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
H11-mScarletLAMA4
Plasmid#172439PurposeHipp11 targeting vector, expresses mScarlet-mLAMA4DepositorInsertmScarlet-mLAMA4
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1N-mScarlet-LZ-LOV2
Plasmid#197419PurposeMammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1DepositorInsertMAPRE1 (MAPRE1 Human)
UseTagsA. sativa phototropin 1 LOV2 domain, GCN4 leucine…ExpressionMammalianMutationEB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…PromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_IRES-mCherry
Plasmid#197427PurposeHomology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5DepositorInsertIRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-mCherry
Plasmid#197429PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsGCN4 leucine zipper, LOV2, Zdk1, and mCherryExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-π-EB1_EF1α-EGFP
Plasmid#197430PurposeHomology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5DepositorInsertEF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
UseCRISPR and Synthetic BiologyTagsEGFP, GCN4 leucine zipper, LOV2, and Zdk1ExpressionMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3 V41A / W43A
Plasmid#113515PurposeExpression of His-I3 V41A / W43 A in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationV41A /W43APromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAG-CRErec
Plasmid#119971PurposeExpresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE proteinDepositorInsertGAG-nlsCRErec
UseTagsnlsExpressionMammalianMutationPromoterhCMVAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only