Skip to main content
Addgene
Showing: 1601 - 1650 of 15599 results

  3. scFLARE1-p2A-eGFP

    Plasmid
    #158699
    Purpose
    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker
    Depositor
    Alice Ting
    Article
    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p
    Insert
    scFLARE1-p2A-eGFP
    Use
    AAV
    Tags
    Flag, V5. eGFP
    Expression
    Mammalian
    Mutation
    Truncated form of TEV protease (220-242 amino aci…
    Promoter
    CMV
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158699-plasmid-map-sequence-id-315073

    Enlarge

  8. mCit(S1-S3)3-SAH

    Plasmid
    #228067
    Purpose
    Mammalian expression of coiled coil segments (S1-S3)x3, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCitrine, S1, S3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228067-plasmid-map-sequence-id-451397

    Enlarge

  9. TagBFP(S2)3(S4)3-gs

    Plasmid
    #228068
    Purpose
    Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2, S4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228068-plasmid-map-sequence-id-451171

    Enlarge

  10. CaMKIV-dCT

    Plasmid
    #126422
    Purpose
    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Article
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Insert
    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126422-plasmid-map-sequence-id-249664

    Enlarge

  11. pFEndoNA2

    Plasmid
    #174667
    Purpose
    Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.
    Depositor
    Jukka Finne
    Article
    Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149
    Insert
    GFP-endoNA2 fusion protein
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available since
    Sept. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    174667-plasmid-map-sequence-id-343065

    Enlarge

  14. MmSphk2

    Plasmid
    #118598
    Purpose
    Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice
    Depositor
    Kevin Lynch
    Article
    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do
    Insert
    Mus musculus Sphingosine Kinase 2 (Sphk2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    GAL1, 10
    Available since
    Sept. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    118598-plasmid-map-sequence-id-236037

    Enlarge

  15. pSOMA-T679A-NLS

    Plasmid
    #118938
    Purpose
    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118938-plasmid-map-sequence-id-232110

    Enlarge

  19. pSOMA-T679A-NES

    Plasmid
    #118940
    Purpose
    Used to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana
    Depositor
    Patrick Krysan
    Article
    Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1
    Insert
    SOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    118940-plasmid-map-sequence-id-232102

    Enlarge

  25. pLV-SFFV-RTN3-WPRE-UbC-Emerald

    Plasmid
    #233467
    Purpose
    Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    RTN3 (RTN3 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    233467-plasmid-map-sequence-id-465704

    Enlarge

  26. pLV-SFFV-myc-ATL3-WPRE-UbC-Emerald

    Plasmid
    #225946
    Purpose
    Lentiviral vector plasmid expressing human atlastin GTPase 3 (ATL3) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    ATL3 (ATL3 Human)
    Emerald
    Use
    Lentiviral
    Tags
    myc-tag
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225946-plasmid-map-sequence-id-446845

    Enlarge

  27. pLV-SFFV-CKAP4 C100A-WPRE-UbC-Emerald

    Plasmid
    #225949
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutation C100A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    C100A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225949-plasmid-map-sequence-id-446851

    Enlarge

  28. pLV-SFFV-KTN1-WPRE-UbC-Emerald

    Plasmid
    #225941
    Purpose
    Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    KTN1 (KTN1 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225941-plasmid-map-sequence-id-446792

    Enlarge

  29. mCit(P5f-P13f)3-gs

    Plasmid
    #228080
    Purpose
    Mammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, P5f, P13f
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228080-plasmid-map-sequence-id-454693

    Enlarge

  30. mCit(S1h)3(S3h)3-gs

    Plasmid
    #228079
    Purpose
    Mammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228079-plasmid-map-sequence-id-454689

    Enlarge

  31. TagBFP(S2-S4)1-gs

    Plasmid
    #228074
    Purpose
    Mammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2, S4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228074-plasmid-map-sequence-id-454672

    Enlarge

  32. TagBFP(S2)3(S4)3-SAH

    Plasmid
    #228069
    Purpose
    Mammalian expression of coiled coil segments (S2)x3-(S4)x3, with long rigid linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2, S4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228069-plasmid-map-sequence-id-454671

    Enlarge

  33. mCit(S1h-S3h)3-gs

    Plasmid
    #228076
    Purpose
    Mammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, S1h, S3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    228076-plasmid-map-sequence-id-454655

    Enlarge

  34. mCit(D1h-D3h)3-gs

    Plasmid
    #228088
    Purpose
    Mammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCit, D1h, D3h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228088-plasmid-map-sequence-id-451399

    Enlarge

  35. TagBFP(S2)2(S4)2-gs

    Plasmid
    #228072
    Purpose
    Mammalian expression of coiled coil segments (S2)x2-(S4)x2, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2, S4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    228072-plasmid-map-sequence-id-451401

    Enlarge

  36. mCit(S1-S3)1-SAH

    Plasmid
    #228073
    Purpose
    Mammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCitrine, S1, S3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228073-plasmid-map-sequence-id-451384

    Enlarge

  38. mCit(S1-S3)3-gs

    Plasmid
    #228070
    Purpose
    Mammalian expression of coiled coil segments (S1-S3)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    mCitrine, S1, S3
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228070-plasmid-map-sequence-id-451165

    Enlarge

  39. TagBFP(S2-S4)3-gs

    Plasmid
    #228075
    Purpose
    Mammalian expression of coiled coil segments (S2-S4)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2, S4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228075-plasmid-map-sequence-id-451136

    Enlarge

  40. TagBFP(S2h)3(S4h)3-gs

    Plasmid
    #228077
    Purpose
    Mammalian expression of coiled coil segments (S2h)x3-(S4h)x3, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.
    Depositor
    Roman Jerala
    Article
    Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:
    Insert
    BFP, S2h, S4h
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    228077-plasmid-map-sequence-id-451130

    Enlarge

  42. pNB_EA_R1CiM_4EGFP_R3EGFPi2

    Plasmid
    #185968
    Purpose
    Molecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.
    Depositor
    Sangram Bagh
    Article
    Mukhopadhyay et al Biotechnol Bioeng. 2020 May;117(5):1502-
    Insert
    Molecular IMPLY gate
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    185968-plasmid-map-sequence-id-370628

    Enlarge

  45. pMT2 Cav2.2 DomI and DomII

    Plasmid
    #89891
    Purpose
    Mammalian expression of Cav2.2 DomI and DomII, this contains the first 2 domains (amino acids 1 -1154) of Cav2.2
    Depositor
    Annette Dolphin
    Article
    Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.
    Insert
    Cacna1b (CACNA1B )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Stop codon after amino acid 1154 (this is a trunc…
    Promoter
    adenovirus major late promoter
    Available since
    April 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    89891-plasmid-map-sequence-id-176913

    Enlarge

  46. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Use
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

    Enlarge

  48. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

    Enlarge

  49. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

    Plasmid
    #225947
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S3E S17E S19E
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225947-plasmid-map-sequence-id-446849

    Enlarge

  50. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid
    #225948
    Purpose
    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Depositor
    Bart Nieuwenhuis, Evan Reid, Richard Eva
    Inserts
    CKAP4 (CKAP4 Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    S3A S17A S19A
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    225948-plasmid-map-sequence-id-446850

    Enlarge

Showing: 1601 - 1650 of 15599 results