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Plasmid#91019PurposeModule A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_H840A nickase
UseCRISPRTagsExpressionMutationH840APromoter35SAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ncerulean-PHdomain
Plasmid#66784PurposeExpression of PIP2 binding protein in sea urchin embryosDepositorInsertPLCdelta (PLCD1 Human)
UseSea urchin, zebrafish, xenopusTagsCeruleanExpressionMammalianMutationonly contains PH domainPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTC303
Plasmid#91210Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
NmCitrine-PHdomain
Plasmid#66783PurposeExpression of PIP2 binding protein in sea urchin embryosDepositorInsertPLCdelta (PLCD1 Human)
UseSea urchin, zebrafish, xenopusTagsmCitrineExpressionMammalianMutationonly contains PH domainPromoterSP6Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p230-pOsAct1::hpt-pZmUbi::BdPetC
Plasmid#129649PurposeRieske FeS overexpressionDepositorInsertCDS of PetC gene encoding for the Rieske FeS subunit
UseTagsExpressionPlantMutationCodon modified for Golden GatePromoterpZmUbiAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_3
Plasmid#91167PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:barDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG484
Plasmid#91194PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)DepositorInsertnTaCas9_H840A+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3306
Plasmid#91102PurposeModule C, Promoter: FMV 34S, Gene: TALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSPDepositorInsertTALE-VP64 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterFMV 34SAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1010
Plasmid#91030PurposeModule A, Promoter: ZmUbi, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterZmUbiAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1002
Plasmid#91029PurposeModule A, Promoter: AtUbi10, Gene: TALEN_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALEN_2)DepositorInsertTALEN_1 backbone with Esp3I ccdb cassette for repeat cloning
UseTALENTagsExpressionMutationPromoterAtUbi10Available sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-H840A_5
Plasmid#91172PurposeCas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_6
Plasmid#91171PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:barDepositorInsertTaCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-D10A_3
Plasmid#91161PurposeCas9 only expression, Cas9 Type: AtCas9_D10A (nickase), Plant Selection: 2x35S:barDepositorInsertAtCas9_D10A (nickase)
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1410
Plasmid#91035PurposeModule A, Promoter: ZmUbi, Gene: TaCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertTaCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterZmUbiAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC440
Plasmid#91225Purposeprotoplast vector expressing gRNA38 targeting barley MLO (control without TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC441
Plasmid#91218Purposeprotoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC436
Plasmid#91222Purposeprotoplast vector expressing gRNA38 targeting barley MLO (with TREX2 expression)DepositorInsertgRNA targeting barley MLO
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC437
Plasmid#91219Purposeprotoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)DepositorInsertgRNAs targeting MLO gene in barley
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAH750
Plasmid#91212Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters, structurally optimized gRNA scaffolds)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-SUMO-B'-l2-B'
Plasmid#209119PurposeBacterial expression of CC-GEMS ligand SUMO-B'-l2-B'DepositorInsertSUMO-B'-l2-B'
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only