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Plasmid#121043PurposeMoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.DepositorInsertKan destination vector with ColE1 origin and mScarlet selection
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C83m
Plasmid#120994PurposeMoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpir protein required for R6K ori
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C24m
Plasmid#120964PurposeMoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.DepositorInsertlambda-cI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C27m
Plasmid#120965PurposeMoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInserttetR (no deg tags)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C98m
Plasmid#121002PurposeMoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.DepositorInsertGp2 toxin
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C51m
Plasmid#120975PurposeMoClo golden gate assembly CD part for sfYFP (superfolder YFP; variant of https://www.fpbase.org/protein/yfp/ - Please see Supplemental Documents for annotated Genbank fileDepositorInsertsfYFP (has BbsI site)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C33m
Plasmid#120968PurposeMoClo golden gate assembly CD part for AiiA (AHL degradase; see doi: [10.1126/science.aaa3794]). Please see Supplemental Documents for annotated Genbank file.DepositorInsertAiiA degradase (Bennett, no ssra tag)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P57m
Plasmid#120949PurposeMoClo golden gate assembly AB part for pCin* (Adam Meyer's improved pCin promoter from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).DepositorInsertpCin Adam Meyer
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
T18m
Plasmid#121037PurposeMoClo golden gate assembly DE part for L3S2P21 (382x-strength synthetic terminator with BsaI cut site removed; see DOI: 10.1038/nmeth.2515).DepositorInsertmodified L3S2P21 (synthetic Voigt terminator)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
V35m
Plasmid#121042PurposeMoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.DepositorInsertKan destination vector with ColE1 origin and GFP selection
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only