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Plasmid#138317PurposeLenti expression of SpyCas9 with Puro selectionDepositorInsertSpyCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFW11-ffluc
Plasmid#128487PurposeAllelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imagingDepositorInsertfirefly luciferase gene
UseAllelic replacement vectorTagsspy0535 downstream flanking region and spy0535 up…ExpressionMutationPromotersyntheticAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_124
Plasmid#133461PurposeEF1a promoter expresses SpyoCas9 and P2A provides EGFPDepositorInsertSpyoCas9
UseCRISPR and LentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1054
Plasmid#71822PurposeExpress Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR1003
Plasmid#71821PurposeExpress Streptococcus pyogenes dCas9 (D10A, H840A)DepositorInsertT7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCR1055
Plasmid#71823PurposeExpress Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLSDepositorInsertT7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCF820_U6-sgRNA-EF1a-mCherry2_lenti
Plasmid#138316PurposeLenti expression of mCherry2 with U6 promoter for SpyCas9 sgRNA targeting GFPDepositorInsertSpyCas9 GFP sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-H2-18 Light chain
Plasmid#216308PurposeExpresses anti-HER2 H2-18 Light chain in mammalian cells. To be paired with H2-18 heavy chain to form the anti-HER2 H2-18 FabDepositorInsertH2-18 Light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianMutationPromoterT7 promoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80l AEC
Plasmid#196473PurposeProtein expression of SpyTag-ELP-CarHCDepositorInsertAEC
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
InterTag-3xL-Δtag
Plasmid#186914PurposeSleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance geneDepositorInsertsfCherry11-(GGGGS)3-PDGFR
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only