We narrowed to 7,620 results for: PAC

1. Epac-S-H170

   Plasmid

   #170352

   Purpose

   mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

   Insert

   mTurq2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGFP-C2-hSphK2

   Plasmid

   #84370

   Purpose

   Expression of AcGFP-SphK2 fusion protein in mammalian cells

   Depositor

   Anthony Don, Hugh Rosen

   Article

   Don et al Biochem Biophys Res Commun. 2009 Feb 27;

   Insert

   SPHK2 (SPHK2 Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pL-SFFV.Reporter.RFP657.PAC

   Plasmid

   #61395

   Purpose

   Lentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. msEGFP-PACR_pcDNA3

   Plasmid

   #55774

   Purpose

   Genetically encoded caged Ca2+

   Depositor

   Takeharu Nagai

   Article

   Fukuda et al ACS Chem Biol. 2014 May 16;9(5):1197-203

   Insert

   PACR
   

   Tags

   msEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 3, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pAct:dCas9-GCN4

   Plasmid

   #78899

   Purpose

   Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-10XGCN4
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Promoter

   pActin (Drosophila)

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Epac-S-H21

   Plasmid

   #170335

   Purpose

   CFP(ST)_EPAC full length_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

   Insert

   CFP(ST)_EPAC full length_Y(UST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Promoter

   yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcUW51-gEgIL

   Plasmid

   #11622

   Depositor

   Pamela Bjorkman

   Article

   Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

   Insert

   HSV-1 glycoprotein E and HSV-1 glycoprotein I
   

   Tags

   His

   Expression

   Insect

   Mutation

   Dicistronic vector containing HSV-1 gE (KOS strai…

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pAC1817_pCR8-gSMN2-EX

   Plasmid

   #118651

   Purpose

   Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-EX
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Epac-S-H30

    Plasmid

    #170337

    Purpose

    CFP(UST)_EPACdDEPCD_Y(UST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Ponsioen et al EMBO Rep. 2004 Dec . 5(12):1176-80.

    Insert

    CFP(UST)_EPACdDEPCD_Y(UST) (RAPGEF3 Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available Since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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