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pBEAST_J23101-amidase

Plasmid

#128135

Purpose

The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

amidase

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_K227/230R_Keima

Plasmid

#127146

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

K227R/K230R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_UNK_G1

Plasmid

#127118

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA UNK (UNK Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBT271.361

Plasmid

#127846

Purpose

clone A3A-W98Y CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

A3A-W98Y

Use

Tags

Expression

Mutation

W98Y

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pKD288

Plasmid

#125075

Purpose

Expression of SO_4638 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4638 (SO_4638 )
lacI (lacI )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR2KN0055

Plasmid

#117622

Purpose

Level2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-12-C

Plasmid

#124693

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-9-G

Plasmid

#124692

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-6-C,16-C

Plasmid

#124694

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-miSFIT-10-G, 15-C

Plasmid

#124695

Purpose

Tuning expression of EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE420:pNEO_CAC1_Vn_HOG1 terminator

Plasmid

#122701

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; N-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

N-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Tags

Expression

Mutation

Promoter

Available since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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YSBE418:pNAT_ACA1_Vc_HOG1 terminator

Plasmid

#122700

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; C-terminal fragment of Venus protein

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

C-terminal fragment of Venus protein

Use

Tagging proteins in cryptococcus neoformans

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17(∆77-91)

Plasmid

#121516

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Use

Tags

Expression

Yeast

Mutation

deleted amino acids 77-91

Promoter

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCu-MCP-2yeGFP(405)

Plasmid

#121419

Purpose

Expressing MCP-2yeGFP

Depositor

Daniel Klionsky

Article

Gatica et al Mol Cell. 2019 Jan 17;73(2):314-324.e4.

Insert

CUP1p-MCP-2yeGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1 promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMV306-ribo-Sec-APEX2

Plasmid

#111701

Purpose

Theophylline-inducible expression of periplasmic APEX2 from a mycobacterial shuttle plasmid (integrating)

Depositor

Jessica Seeliger

Article

Ganapathy et al ACS Infect Dis. 2018 Apr 30. doi: 10.102

Insert

APEX2

Use

Tags

V5 and mpt63 signal sequence

Expression

Bacterial

Mutation

Promoter

hsp60-ribo

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0023

Plasmid

#117538

Purpose

Level1 Golden Gate Cassette: extended stem sgRNA cassette with AtU6-26 terminator for SpCas9, targeting NbPDS gene in N. benthamiana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

sgRNA (Sp Cas9) - extended stem with U626 terminator

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pVL21 - tVND7

Plasmid

#116028

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tVND7

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL13 - tNST3

Plasmid

#116027

Purpose

entry vector for GreenGate cloning method, terminator

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

tNST3

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL28 - pSMXL5

Plasmid

#116025

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pSMXL5

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pVL36 - pAt2g38380

Plasmid

#116023

Purpose

entry vector for GreenGate cloning method, promoter

Depositor

Thomas Greb

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAt2g38380

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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