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Plasmid#91162PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM1162
Plasmid#112535PurposepZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
UseTagsExpressionBacterialMutationPromoterJ23107 and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_OCT4 3UTR vector
Plasmid#99295PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Plxnb2-ISH-2kb
Plasmid#68036PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertPlexin-B2 (Plxnb2 Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
JPF0454
Plasmid#124039PurposeEncodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-spCAS9-P2A-mAzamiGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV BARK p2A mCherry
Plasmid#117689PurposeCMV-iBARK-p2A-mCherry: Mammalian expression vector for Gaq silencingDepositorInsertBARKrgs peptide
UseTagsHA / FLAGExpressionMammalianMutationPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_1
Plasmid#91165PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_mCP vector
Plasmid#99291PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorInsertERGIC2-WT (ERGIC2 Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCRII-Sema4c-ISH-2kb
Plasmid#68031PurposeContains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouseDepositorInsertSemaphorin-4C (Sema4c Mouse)
UseMrna in situ hybridization probeTagsExpressionMutationPromoterAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0802
Plasmid#91023PurposeModule A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_dead (D10A + H840A)
UseCRISPRTagsExpressionMutationD10A, H840APromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN1779
Plasmid#127165PurposeVector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)DepositorInsertpGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-miniSOG-C-Src-7
Plasmid#55086PurposeLocalization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertC-Src
UseTagsmCherry-miniSOGExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rat SERCA3 (pMT2)
Plasmid#75185PurposeMammalian expression of ATP2A3DepositorInsertATP2A3 (Atp2a3 Rat)
UseTagsExpressionMammalianMutationPromoteradenovirus major late promoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJG493
Plasmid#91197PurposeWDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)DepositorInsertgUbi1+gUbi8+donor
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK234_027
Plasmid#123907PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorTagsExpressionInsectMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9_5
Plasmid#91168PurposeCas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_2
Plasmid#91163PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRTagsExpressionPlantMutationH840APromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only