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pXPR_003 sgMSLN

Plasmid

#193588

Purpose

MSLN knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMSLN (MSLN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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HI.fate.UB

Plasmid

#191556

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV-E

Plasmid

#191555

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.E

Plasmid

#191553

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV

Plasmid

#191554

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Tags

Expression

Mutation

env-deleted + vpr mutation(s)

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW1,2,4

Plasmid

#171137

Purpose

expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L (NEDD4L Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 221 to alanine, tryptophan 453…

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSICO-CPSF6-4Glu-iRFP670

Plasmid

#167588

Purpose

Expresses a mutant form of CPSF6 (CPSF6-4Glu described in Jang et al. Nucleic Acids Res 2019) fused to iRFP670

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-4Glu-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Expression

Mutation

K547E, R549E, R559E, R561E

Promoter

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS2271

Plasmid

#158418

Purpose

AAV plasmid with Ple341 (PCP2 MiniPromoter) driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple341 (PCP2 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Ple341

Available since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PTPRA-MAC

Plasmid

#139632

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA (PTPRA Human)

Use

Tags

StrepIII-HA-BirA*

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57mini ZM249M P90A _env eGFP

Plasmid

#132939

Purpose

pUC57mini ZM249M _env eGFP containing P90A mutant capsid sequence

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

HIV-1ZM249M provirus (P90A) with env deletion, GFP

Use

Lentiviral

Tags

Expression

Mutation

P90A

Promoter

Available since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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CMV 197 E7

Plasmid

#85030

Purpose

HPV 197 E7 in CMV

Depositor

Karl Munger

Article

Grace et al Virology. 2016 Oct 20;500:71-81. doi: 10

Insert

HPV 197 E7

Use

Tags

Expression

Mammalian

Mutation

Codon optimized for expression

Promoter

CMV

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Cdk6-HA

Plasmid

#1868

Depositor

Sander van den Heuvel

Article

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Insert

Cdk6 (CDK6 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D224Y

Promoter

Available since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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gGBEv6.3-SpCas9

Plasmid

#202629

Purpose

vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

Insert

nSpCas9(D10A)-MPGv6.3

Use

Tags

Expression

Mammalian

Mutation

MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

Promoter

hU6, EF1a, CBh

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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YFP-tagged FRB (YR)

Plasmid

#20148

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FRB (YR)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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dRED

Plasmid

#139836

Purpose

Expresses dRNase H1-EGFP-dCas9 fusion protein in human cells

Depositor

Karim Mekhail

Article

Abraham et al Nature. 2020 Sep;585(7824):298-302. doi:

Insert

dRNase H1-EGFP-dCas9 (RNASEH1 dRNase H1 (H. sapiens), dCas9 (S. pyogenes))

Use

Tags

Expression

Mammalian

Mutation

RNase H1 protein contains D210N mutation. dCas9 …

Promoter

tet-inducible CMV

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_TauFRET_3xKQ_wpre

Plasmid

#207522

Purpose

Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

Depositor

Bradley Hyman

Article

Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

Insert

TauFRET_P301L_3xKQ

Use

Lentiviral

Tags

NeonGreen and mTurquoise2

Expression

Mutation

P301L; K311Q; K317Q; K321Q

Promoter

EF1a

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHSP02

Plasmid

#117259

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

Cas9, promotor P11-guide-RNA, homologous arms of Lp_0537

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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phND2-N106

Plasmid

#31780

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

NeuroD2 (NEUROD2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn1-GRAB-HTR6-cilia

Plasmid

#189615

Purpose

Expresses cilia-targeted serotonin sensor based on HTR6 receptor and cpEGFP, driven by SYN promoter

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6_GRAB

Use

AAV

Tags

Expression

Mutation

Promoter

Syn1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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AAV CMV-dSaCas9-KRAB-bGHpA

Plasmid

#106219

Purpose

AAV expressing deactivated S. aureus Cas9 fused to a KRAB repressor

Depositor

Charles Gersbach

Article

Thakore et al Nat Commun. 2018 Apr 26;9(1):1674. doi:

Insert

dead S. aureus Cas9 KRAB

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Mutation

D10A and N580A

Promoter

CMV

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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