Addgene

We narrowed to 15,735 results for: puromycin

Showing: 6541 - 6560 of 15735 results

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pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
pBabe-Neuroserpin oloop

Plasmid

#58260

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Promoter

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
tet-pLKO shMKP7.v1 puro

Plasmid

#87794

Purpose

Expresses an inducible short hairpin targeting human MKP7 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP7 (DUSP16 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shDUSP19 puro

Plasmid

#87796

Purpose

Expresses an inducible short hairpin targeting human DUSP19 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shDUSP19 (DUSP19 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001146785)

Plasmid

#77412

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001148893)

Plasmid

#77413

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ICK gRNA (BRDN0001146903)

Plasmid

#77414

Purpose

3rd generation lentiviral gRNA plasmid targeting human ICK

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ICK

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiX2-PURO-shDIXDC1-Ms-57

Plasmid

#61232

Purpose

Lentivirus expressing shRNA to mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

Dixdc1 (Dixdc1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF_S214F

Plasmid

#51078

Purpose

mammalian expression of ARAF S214F

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S214F

Promoter

Available since

Feb. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA SOX17 -296

Plasmid

#50926

Purpose

U6 driven sgRNA targeting Sox17 -296 bp from TSS

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

Sox17-296 sgRNA (SOX17 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE Gal4(1-94) HSF ABC Flag ph

Plasmid

#1947

Depositor

Robert Kingston

Article

Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

Insert

Heat Shock Factor 1 (HSF1 Human)

Use

Retroviral

Tags

Gal4 DBD and flag

Expression

Mammalian

Mutation

phenylalanine mutant (F418, F492, F500 A)

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pPB9v2

Plasmid

#210029

Purpose

Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB758

Plasmid

#210033

Purpose

Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas7-P2A-Cas5-T2A-Cas8
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB12A

Plasmid

#210035

Purpose

Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas12a
rtta-T2A-puro

Use

CRISPR

Tags

nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9

Plasmid

#210036

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
puro-T2A-eGFP

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9v1

Plasmid

#210028

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgMTHFD1

Plasmid

#217436

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human MTHFD1

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting MTHFD1 (MTHFD1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTYMS

Plasmid

#217430

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TYMS

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA targeting TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_4

Plasmid

#217431

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 4 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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