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Plasmid#126554PurposeBroad host range plasmid derived from pBBR1-MCS5 with pemI/pemK toxin-antitoxin module from Xylella fastidiosa to improve stability in the absence of antibiotic selectionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only
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pDCC6
Plasmid#59985PurposeBi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9 and U6>sgRNA cassetteDepositorInsertsCas9
U6-2>sgRNA
UseCRISPRTags3xFLAGExpressionMutationPromoterU6-96Ab and hsp70BbAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAT30
Plasmid#204294PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCM18
Plasmid#78565Purpose60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmidDepositorTypeEmpty backboneUseTagsluxCDABEExpressionBacterialMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJAT17
Plasmid#204298PurposeFor introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.DepositorInsert3XP3-EYFP
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFG036
Plasmid#137996PurposecI repressor expression plasmidDepositorInsertcI
UseSynthetic BiologyTagsExpressionBacterialMutationThermosensitivePromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UbC-mCherry Rab5
Plasmid#115239PurposeTo express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.DepositorInsertRab5a (RAB5A Human, Homo sapiens)
UseAAVTagsmCherryExpressionMammalianMutationPromoterHuman UbCAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
3xDBE_RE:NLuc
Plasmid#178318PurposeFOXO NLuc luciferase reporterDepositorInsertFOXO NLuc Reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSelAct-KO
Plasmid#174374PurposePlasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatibleDepositorTypeEmpty backboneUseVector for 2-step knockout by homologous recombin…TagsExpressionMutationPromoterN/AAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
7MEQ
Plasmid#176412PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertTMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
UseBaculovirus expresssionTags8 x His Tag and Honeybee melittin signalExpressionMutationetmprss2_S251D/R252D/Q253D/S254D/R255KPromoterPolyhedrinAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_RE:RedF
Plasmid#178314PurposeHRE RedFirefly luciferase reporterDepositorInsertHRE RedFirefly reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGW-GOI-TurboID-GFP
Plasmid#209379PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT62
Plasmid#204301PurposeExpress phiC31 integrase under heat shock control. Insert marked with ie1-EGFP, which expresses in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT11
Plasmid#204290PurposeFor inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.DepositorInsertie1-DsRed
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRDX6A-c001
Plasmid#175474PurposeProtein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.DepositorInsertPRDX6 (PRDX6 Human)
UseTagsAviTag and His6-TEVExpressionBacterialMutationSee Depositor CommentsPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGW-GFP-TurboID-GOI
Plasmid#209381PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ211_WT_low
Plasmid#128848PurposeLow copy number plasmid for expression of E. coli flagellin from its natural promoterDepositorInsertFlagellin
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAT43
Plasmid#204299PurposeFor introducing heat shock inducble B3 recombinase. HDR integrant marked with ie1-EGFP, expressed in abdomen and mouthparts.DepositorInsertie1-EGFP
UseCRISPRTagsExpressionMutationPromoterie1Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGW-TurboID-GOI-GFP
Plasmid#209383PurposeGateway compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneUseTagsExpressionPlantMutationTurboID gene sequence C249APromoterAvailable sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH3FS
Plasmid#85780PurposeN-ICE plasmid pH3FSDepositorInsertloxP-HphMX-loxP-3FLAG
UseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only