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Plasmid#138819PurposeConstitutive expression of VP64-ZF43(RAAR) under the CMV promoter.DepositorInsertVP64-ZF43(RAAR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD113 VP16-ZF43(AAAA)
Plasmid#138818PurposeConstitutive expression of VP16-ZF43(AAAA) under the CMV promoter.DepositorInsertVP16-ZF43(AAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43A, R71APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD112 VP16-ZF43(AAAR)
Plasmid#138817PurposeConstitutive expression of VP16-ZF43(AAAR) under the CMV promoter.DepositorInsertVP16-ZF43(AAAR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15A, R43APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD111 VP16-ZF43(AARR)
Plasmid#138816PurposeConstitutive expression of VP16-ZF43(AARR) under the CMV promoter.DepositorInsertVP16-ZF43(AARR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6A, R15APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD110 VP16-ZF43(ARRR)
Plasmid#138815PurposeConstitutive expression of VP16-ZF43(ARRR) under the CMV promoter.DepositorInsertVP16-ZF43(ARRR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR6APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD109 VP16-ZF43(RAAA)
Plasmid#138814PurposeConstitutive expression of VP16-ZF43(RAAA) under the CMV promoter.DepositorInsertVP16-ZF43(RAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43A, R71APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD108 VP16-ZF43(RAAR)
Plasmid#138813PurposeConstitutive expression of VP16-ZF43(RAAR) under the CMV promoter.DepositorInsertVP16-ZF43(RAAR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43APromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIE059 ZF43x1 mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138740PurposeIntegration Vector, assembled with pHIE043 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertZF43x1
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIE063 ZF43x3-S mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138744PurposeIntegration Vector, assembled with pHIE047 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertZF43x3-Spaced
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIE064 ZF43x6-S mKate2 EF1a EBFP2-P2A-BlastR
Plasmid#138745PurposeIntegration Vector, assembled with pHIE048 (TUPV1), pJM451 (TUPV2), pPD612DepositorInsertZF43x6-Spaced
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJM466 EF1a VP16-ZF43 (TUPV2)
Plasmid#138730PurposemMoClo TUPV, with EF1alpha promoter and VP16-ZFa in the MCSDepositorInsertVP16-ZF43
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWD95
Plasmid#158617PurposeCckA FRET SensorDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationPromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_4E1H.95
Plasmid#155213PurposeExpresses 4E1H.95 de novo protein in E.coliDepositorInsert4E1H.95
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_4H.01
Plasmid#155210PurposeExpresses 4H.01 de novo designed protein in E.coliDepositorInsert4H.01
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
4E1H.95_LUMABS
Plasmid#155208PurposeExpresses 4E1H.95_LUMABS sensor in E.coliDepositorInsert4E1H.95_LUMABS
UseTags6x His TagExpressionMutationPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_3E2H.37
Plasmid#155211PurposeExpresses 3E2H.37 de novo designed protein in E.coliDepositorInsert3E2H.37
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET_3H1L_02.395
Plasmid#155198PurposeExpresses 3H1L_02.395 in E.coliDepositorInsert3H1L_02.395
UseTags6x His TagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer
Plasmid#138365PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence.DepositorInsert161k human enhancer
UseGateway compatibleTagsExpressionMutation9 A bases deletion, that have no impact on the en…PromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pst39-NST-clone14
Plasmid#133421PurposeS. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).DepositorUseTagsExpressionBacterialMutationORF for Nip1 encodes aa 4-156 (not complete ORF),…PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only