Addgene

We narrowed to 10,528 results for: GAN

Showing: 6541 - 6560 of 10528 results

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PhyB-mCherry-MYO1

Plasmid

#51570

Purpose

Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PhyB (PHYB Mustard Weed)

Use

Tags

MYO1 and mCherry

Expression

Yeast

Mutation

1-908aa only

Promoter

ADH1pr

Available since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Jag2ICD-HA pBacPAK8-3

Plasmid

#17332

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged2 (Jag2 Rat)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-SLC30A10-N43H

Plasmid

#82344

Purpose

Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

N43H

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

Plasmid

#182358

Purpose

Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

GFP

Use

Tags

Expression

Worm

Mutation

Promoter

Pmex-5

Available since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR2E3_Hormone-recep

Plasmid

#109852

Purpose

Protein expression and purification of NR2E3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR2E3_Hormone-recep (NR2E3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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5mCUC1 pGreenII0129

Plasmid

#12067

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Tags

Expression

Mutation

miR-resistant CUC1. Made by introducing five sile…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-SLC30A10-D248A

Plasmid

#82346

Purpose

Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

Insert

SLC30A10 (SLC30A10 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D248A

Promoter

CMV

Available since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV mTurquoise2-shadowY tandem

Plasmid

#157775

Purpose

Produces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Article

Bertolin et al Elife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Use

Tags

mTurquoise2 and shadowY

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-2

Plasmid

#172739

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-2

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Mutation

Promoter

'long' CaMV35S

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_012

Plasmid

#123967

Purpose

Encodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

hRosa26-BxBI landing pad

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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p7274 MSCV-P C-FlagHA 16 ∆DLYC

Plasmid

#163304

Purpose

Expresses HPV16 E7 ∆DLYC

Depositor

Elizabeth White

Article

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 ∆DLYC

Use

Retroviral

Tags

FlagHA

Expression

Mutation

∆DLYC

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-RatLC3

Plasmid

#55095

Purpose

Localization: Microtubule-Binding Protein, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LC3

Use

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pAtCas9-dead_2

Plasmid

#91166

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, H840A

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NLS

Plasmid

#124625

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-OSBP

Plasmid

#67677

Purpose

Multisite gateway entry clone for adding C-terminal fusions of OSBP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

OSBP

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

aa 87-185 of NP_002547.1

Promoter

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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Jag1ICD-HA pBacPAK8-3

Plasmid

#17331

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged1 (Jag1 Rat)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCG150_double_phiC31_attB

Plasmid

#169693

Purpose

cargo vector for the phiC31 system with, unc-119 rescue fragment, no fluorescent marker. Gateway R3R4 sites (for gene of interest insertion)

Depositor

Shih-Peng Chan

Article

Yang et al Genetics. 2022 Feb 4;220(2):iyab206. doi

Type

Empty backbone

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPN021

Plasmid

#91667

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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