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Showing: 6521 - 6540 of 20471 results

  1. pCMV-ABE-P48R-UGI

    Plasmid
    #161817
    Purpose
    Expresses ABE-P48R-UGI in mammalian cells
    Depositor
    Sangsu Bae
    Insert
    bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-10AA linker-UGI-10AA linker-UGI-bpNLS-P2A-EGFP-NLS
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A in S. pyogenes Cas9, TadA mutations describe…
    Promoter
    Available since
    Jan. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    161817-plasmid-map-sequence-id-320651

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  2. pTEAD1-donor

    Plasmid
    #112329
    Purpose
    CRISPR donor plasmid to tag human transcription factor TEAD1 with GFP
    Depositor
    Kevin White
    Insert
    TEAD1 homology arms flanking EGFP-IRES-Neo cassette (TEAD1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112329-plasmid-map-sequence-id-228556

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  3. pUSF2-donor

    Plasmid
    #112361
    Purpose
    CRISPR donor plasmid to tag human transcription factor USF2 with GFP
    Depositor
    Kevin White
    Insert
    USF2 homology arms flanking EGFP-IRES-Neo cassette (USF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:35278400:T>A, hg38:19:35278455:G>C
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112361-plasmid-map-sequence-id-229684

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  4. p-dCas9-SET7-Hygro

    Plasmid
    #104407
    Purpose
    transient expression of dCas9-SET7 fusion protein
    Depositor
    Stephan Beck
    Insert
    SET7 (SETD7 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104407-plasmid-map-sequence-id-204449

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  6. pUC57-sgDedd-1

    Plasmid
    #74435
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 5
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. Cas9-T2A-H2B mCitirine

    Plasmid
    #210471
    Purpose
    Coexpression of Cas9 and H2B mCitrine
    Depositor
    Amro Hamdoun
    Insert
    Cas9-T2A-H2B mCitirine
    Use
    CRISPR and Synthetic Biology
    Tags
    mCitrine on C terminal of H2B
    Expression
    Mammalian
    Mutation
    Cas9 contains 3xFLAG tag and nuclear localization…
    Promoter
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    210471-plasmid-map-sequence-id-416284

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  8. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

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  9. pNR2F1-donor

    Plasmid
    #112388
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR2F1 with GFP
    Depositor
    Kevin White
    Insert
    NR2F1 homology arms flanking EGFP-IRES-Neo cassette (NR2F1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs974916569
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112388-plasmid-map-sequence-id-229852

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  10. pZNF250-donor

    Plasmid
    #112383
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF250 with GFP
    Depositor
    Kevin White
    Insert
    ZNF250 homology arms flanking EGFP-IRES-Neo cassette (ZNF250 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:144882271:C>G,hg38:8:144882273:T>C
    Promoter
    Available since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    112383-plasmid-map-sequence-id-229838

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  11. pZNF140-donor

    Plasmid
    #112363
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF140 with GFP
    Depositor
    Kevin White
    Insert
    ZNF140 homology arms flanking EGFP-IRES-Neo cassette (ZNF140 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:133105834:A>G, hg38:12:133105852:A>G
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112363-plasmid-map-sequence-id-229689

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  12. pZNF639-donor

    Plasmid
    #112345
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF639 with GFP
    Depositor
    Kevin White
    Insert
    ZNF639 homology arms flanking EGFP-IRES-Neo cassette (ZNF639 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:3:179333542:G>T,rs978050614
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112345-plasmid-map-sequence-id-229659

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  13. pTBPL1-donor

    Plasmid
    #112316
    Purpose
    CRISPR donor plasmid to tag human transcription factor TBPL1 with GFP
    Depositor
    Kevin White
    Insert
    TBPL1 homology arms flanking EGFP-IRES-Neo cassette (TBPL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    rs35776966, hg38:6:133987646:GTGTAT>-
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112316-plasmid-map-sequence-id-228480

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  14. pUC57-sgDedd-2

    Plasmid
    #74436
    Purpose
    sgRNA expression vector for mouse Dedd gene targeting intron 4
    Depositor
    Petra Arck
    Insert
    Dedd (Dedd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  20. pJK511

    Plasmid
    #155384
    Purpose
    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus
    Depositor
    Pamela Silver
    Article
    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81
    Inserts
    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D917A (nuclease-deactivating)
    Promoter
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    155384-plasmid-map-sequence-id-309004

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Showing: 6521 - 6540 of 20471 results