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Plasmid#208870PurposeFor expression in E. coli of GFP-NAP1DepositorInsertNAP1 (NAP1L1 Human)
UseTagsGFP and GST-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual- GST-TEV-TBK1
Plasmid#208875PurposeFor expression in insect cells of TBK1DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-NAP1
Plasmid#208862PurposeStably express GFP-tagged NAP1 for CID induced mitophagyDepositorInsertNAP1 (NAP1L1 Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPIBY55
Plasmid#213720PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIBY55 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPIDAF
Plasmid#213719PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPIDAF under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
psgRNA-GST-EGFP-GPICEAM7
Plasmid#213721PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of the affinity sorting tag GST-EGFP-GPICEAM7 under a CMV promoter.DepositorInsertEGFP
UseCRISPRTagsGSTExpressionMutationPromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS100
Plasmid#209354PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-moxMaple-GW
Plasmid#209353PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-HALO-GW
Plasmid#209351PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-SNAP-GW
Plasmid#209350PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHyg-His6
Plasmid#212776PurposeC-terminal PCR-based tagging with His6 in budding yeast with HygR selectionDepositorTypeEmpty backboneUsePcr-based taggingTagsExpressionMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA106
Plasmid#209339PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA105
Plasmid#209338PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal Dendra2 fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJRA101
Plasmid#209335PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mVenus fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLCS111
Plasmid#209332PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR150APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAAA
Plasmid#202587PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_StammerAEA
Plasmid#202588PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purificationDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Methionine 91 to Alanine, changed O…PromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p_K3AK4A
Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only