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pX459-SpCas9-NG

Plasmid

#171370

Purpose

SpCas9-NG-mediated genome editing.

Depositor

Masahito Ikawa

Article

Oura et al Commun Biol. 2021 Jun 23;4(1):771. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA; CBh for Cas9-2A-puroR

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCTcon2-5INS_EV3

Plasmid

#102293

Purpose

Yeast Surface Display of 5Ins_16_EV3

Depositor

Sarel Fleishman

Article

Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

Insert

Evolved Insulin binding scFV

Use

Tags

3x(G4S) Linker, G3S Linker, HA, and Myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pGEmCherry2

Plasmid

#118413

Purpose

Red fluorescent reporter for assessing CRISPR gRNA activity

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

mCherry

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt6

Plasmid

#35913

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Tags

Expression

Mammalian

Mutation

Silent Mutation G (567) --> A

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf5

Plasmid

#163596

Purpose

Expresses GDF-5 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf5 (Gdf5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_HP1

Plasmid

#82607

Purpose

Expresses sfCherry2(11) tagged heterochromatin protein1 in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_HP1b

Use

Tags

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only
pUC-H1-gRNA

Plasmid

#61089

Purpose

CRISPR/Cas9 system gRNA cloning

Depositor

Shenglin Huang

Article

Zheng et al Biotechniques. 2014 Sep 1;57(3):115-24.

Insert

H1 promoter; gRNA sequences

Use

CRISPR; /cas9 grna cloning vector

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pC008 - pBR322 with tetR-inducible RFP

Plasmid

#79157

Purpose

inducible RFP expression vector

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

TetR + RFP

Use

Tags

Expression

Mutation

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp4

Plasmid

#163588

Purpose

Expresses BMP-4 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp4 (Bmp4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pT2HLacZpA1I.7 (#59)

Plasmid

#35965

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter (Tek Mouse)
SDK LacZ
SV40 pA
Tie2 full enhancer

Use

Tags

Expression

Mutation

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt7A

Plasmid

#35914

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA

Plasmid

#52549

Purpose

transgenic construct for expression of GFP and Flpo

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP
Flpo recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-TagGFP2

Plasmid

#121426

Purpose

TAgGFP2 was amplified from pLenti-Origene-Nrf21 and inserted into XhoI and EcoRI digested pLenti-Origene-Nrf21.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

empty TagGFP2

Use

Lentiviral

Tags

TagGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Hras

Plasmid

#180580

Purpose

ddGFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Hras

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5A-V5

Plasmid

#35930

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3A-V5

Plasmid

#35927

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

April 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

Plasmid

#202569

Purpose

Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

LINE-1

Use

Tags

GFP-AI retrotransposition reporter and HaloTag7 o…

Expression

Mammalian

Mutation

Changed ORF1p Arginine 261 to Alanine

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET21(+)-His6-Ubc13

Plasmid

#212715

Purpose

Bacterial expression of His6-Ubc13

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET30-RT1306

Plasmid

#131521

Purpose

Expresses engineered RT-1306 with His-tag

Depositor

Bryan Dickinson

Article

Zhou et al Nat Methods. 2019 Dec;16(12):1281-1288.

Insert

RT-1306

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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